SPATIAL PATTERNS OF GENE-EXPRESSION IN BRASSICA-NAPUS SEEDLINGS - IDENTIFICATION OF A CORTEX-SPECIFIC GENE AND LOCALIZATION OF MESSENGER-RNAS ENCODING ISOCITRATE LYASE AND A POLYPEPTIDE HOMOLOGOUS TO PROTEINASES
SPATIAL PATTERNS OF GENE-EXPRESSION IN BRASSICA-NAPUS SEEDLINGS - IDENTIFICATION OF A CORTEX-SPECIFIC GENE AND LOCALIZATION OF MESSENGER-RNAS ENCODING ISOCITRATE LYASE AND A POLYPEPTIDE HOMOLOGOUS TO PROTEINASES
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DOI：
10.1105/tpc.1.1.73
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发表时间：
1989-01-01
期刊：
影响因子：
11.6
通讯作者：
HARADA, JJ
中科院分区：
文献类型：
--
作者：
DIETRICH, RA;MASLYAR, DJ;HARADA, JJ
利用原位杂交技术研究了甘蓝型油菜种子萌发和萌发后发育过程中表达的三个基因的空间表达。其中两个mrna编码异柠檬酸裂解酶和一种与半胱氨酸蛋白酶同源的预测多肽。我们之前报道过，这些mrna主要存在于幼苗的子叶中，并在萌发后的生长过程中以类似的动力学积累。在这里，我们发现这两种mrna在几种幼苗组织中都被检测到，但它们在子叶和根冠轴上的分布模式不同。第三种mRNA在幼苗轴中含量丰富，并在地下分生组织、下胚轴和根的成熟皮层中特异性积累。mRNA在根分生组织中的分布表明，该基因产物参与了皮层细胞分化的早期过程。我们的研究结果提供了对幼苗生理过程的深入了解。
We investigated the spatial expression of three genes that are expressed during seed germination and postgerminative development in Brassica napus L. using in situ hybridization procedures. Two of the mRNAs encode isocitrate lyase and a predicted polypeptide that is homologous to cysteine proteinases. We reported previously that the mRNAs are prevalent primarily in cotyledons of seedlings and accumulate with similar kinetics during postgerminative growth. Here, we show that the two mRNAs are detected in several seedling tissues, but they display different distribution patterns in both cotyledons and root-shoot axes. The third mRNA is abundant in seedling axes and accumulates specifically in the ground meristem and mature cortex of hypocotyls and roots. Distribution of the mRNA in root meristems suggests that the gene product participates in an early event in cortical cell differentiation. Our results provide insight into the physiological processes that characterize seedlings.