Comparison of the foetal development of muscle in normal and double-muscled cattle
Comparison of the foetal development of muscle in normal and double-muscled cattle
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正常牛和双肌牛胎儿肌肉发育的比较
DOI：
10.1007/bf00130244
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发表时间：
1995
影响因子：
2.7
通讯作者：
Y. Geay
中科院分区：
文献类型：
--
作者：
B. Picard;H. Gagnière;J. Robelin;Y. Geay
肌肉分化进行了研究，在胎儿半腱肌肌肉从正常牛和那些与'culard'基因的肌肉肥大取样在90，130，170和210天的胎儿生活。不同的纤维类型，其特征在于免疫组织化学与抗体特异性不同亚型的肌球蛋白重链。通过电泳分离亚型，通过免疫印迹鉴定并通过ELISA定量。在双肌动物中，第一代细胞的分化速度较慢，在90天最明显。在130天时，分化主要在第二代中延迟，而在170天时，两种动物类型之间在第一代细胞的分化方面不再有任何差异，第一代细胞的分化在两种动物中都完全缓慢。然而，在同一阶段，双肌动物的IIC型纤维出现得慢得多，并继续在210天，虽然在较小的程度上。这些发现表明，双肌胎儿的肌纤维分化速度较慢，特别是在胎儿生命的前三分之二期间。
Muscle differentiation was studied in foetal Semitendinosus muscle from normal cattle and those with the ‘culard’ gene of muscular hypertrophy sampled at 90, 130, 170 and 210 days of foetal life. The different fibre types were characterized by immunohistochemistry with antibodies specific to different isoforms of myosin heavy chains. The isoforms were separated by electrophoresis, identified by immunoblotting and quantified by ELISA. In double-muscled animals, there was a slower rate of differentiation in the first generation of cells, most markedly apparent at 90 days. At 130 days, differentiation was retarded mainly in the second generation, while at 170 days there was no longer any difference between the two animal types in the differentiation of first generation cells, which were totally slow in both. At the same stage however, type IIC fibres in double-muscled animals were much slower in appearing and continued to be so at 210 days, albeit to a lesser extent. These findings show that differentiation of the muscle fibres occurs at a slower rate in double-muscled foetuses particularly during the first two-thirds of foetal life.