Neuron-immune interactions in the sensitized thalamus induced by mustard oil application to rat molar pulp
Neuron-immune interactions in the sensitized thalamus induced by mustard oil application to rat molar pulp
复制标题
芥末油应用于大鼠磨牙髓诱导致敏丘脑中的神经元免疫相互作用
DOI：
10.1177/0022034510377202
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发表时间：
2010
影响因子：
7.6
通讯作者：
Suda H
中科院分区：
文献类型：
--
作者：
Kawamura J;Kaneko T;Kaneko M;Sunakawa M;Kaneko R;Chokechanachaisakul U;Okiji T;Suda H
我们已经报道，在大鼠牙髓中应用芥子油可以诱导丘脑神经元的激活。为了探讨丘脑变化的机制，我们进行了神经元反应性记录、免疫组织化学和分子生物学分析。应用芥子油后，背内侧核神经元反应性增强。当MK801(ANN-甲基-D-天冬氨酸受体拮抗剂)应用于背内侧核时，增强的反应性降低。对侧丘脑N-甲基-D-天冬氨酸受体2D、胶质纤维酸性蛋白和抗原提呈细胞相关基因mRNAs在芥子油作用后10min上调，但在拮抗剂作用后10min下调。应用芥子油60min后，表达OX6的小胶质细胞和表达胶质纤维酸性蛋白的星形胶质细胞才开始增加。这些结果提示，在牙髓炎症诱导的中枢敏化过程中，丘脑神经元可能通过N-甲基-D-天冬氨酸2D受体参与调节背内侧核神经胶质细胞的激活。
We have reported that mustard oil application to the rat dental pulp induces neuronal activation in the thalamus. To address the mechanisms involved in the thalamic changes, we performed neuronal responsiveness recording, immunohistochemistry, and molecular biological analysis. After mustard oil application, neuronal responsiveness was increased in the mediodorsal nucleus. When MK801 (anN-methyl-D-aspartate receptor antagonist) was applied to the mediodorsal nucleus, the enhanced responsiveness was decreased.N-methyl-D-aspartate receptor 2D, glial fibrillary acidic protein, and antigen-presenting cell-related gene mRNAs in the contralateral thalamus were up-regulated at 10 minutes after mustard oil application, but were down-regulated within 10 minutes after the antagonist application. OX6-expressing microglia and glial fibrillary acidic protein-expressing astrocytes did not increase until 60 minutes after mustard oil application. These results suggested that the thalamic neurons play some roles in regulating the glial cell activation in the mediodorsal nucleusvia N-methyl-D-aspartate receptor 2D during pulp inflammation-induced central sensitization.