High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry
High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry
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DOI：
10.1038/nprot.2007.500
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发表时间：
2008-01-01
期刊：
影响因子：
14.8
通讯作者：
Draper, John
中科院分区：
文献类型：
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作者：
Beckmann, Manfred;Parker, David;Draper, John
流动注射电喷雾质谱法（FIE-MS）作为一种代谢物指纹识别工具在许多研究领域得到了广泛应用。我们提供的方案在涉及来自植物、微生物和哺乳动物生物流体的各种样品基质的大规模研究项目中被证明是可靠的。以短柄草叶片材料为例，总结了从样品提取到数据质量评价的所有步骤。包括处理其他常见基质（如血液和尿液）的替代程序。样品预处理少，无需色谱，仪器循环次数为每周1000个样品。典型批次240个样品的分析（包括第一次数据分析）可在48小时内完成。
Flow injection electrospray-mass spectrometry (FIE-MS) is finding utility as a first-pass metabolite fingerprinting tool in many research fields. We provide a protocol that has proved reliable in large-scale research projects involving diverse sample matrices originating from plants, microbes and mammalian biofluids. Using Brachypodium leaf material as an example matrix all steps are summarized from sample extraction to data quality assessment. Alternative procedures for dealing with other common matrices such as bloods and urine are included. With little sample pretreatment, no chromatography and instrument cycle times of 1,000 samples per week. Analysis of a typical batch of 240 samples (including first-pass data analysis) can be accomplished within 48 h.