M-cell surface β1 integrin expression and invasin-mediated targeting of Yersinia pseudotuberculosis to mouse Peyer's patch M cells
M-cell surface β1 integrin expression and invasin-mediated targeting of Yersinia pseudotuberculosis to mouse Peyer's patch M cells
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DOI：
10.1128/iai.66.3.1237-1243.1998
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发表时间：
1998-03-01
影响因子：
3.1
通讯作者：
Jepson, MA
中科院分区：
文献类型：
--
作者：
Clark, MA;Hirst, BH;Jepson, MA
假结核耶尔森氏菌感染小鼠肠袢的定量分析显示，与Peyer's patch M细胞相关的野生型细菌明显多于与圆顶肠上皮细胞或杯状细胞相关的野生型细菌。侵袭素缺陷突变体的M细胞侵袭显着减弱，而β 1整合素的表达被证明在顶膜的M细胞，但不是肠上皮细胞。因此，假结核耶尔森氏菌在体内的M细胞靶向作用可能主要由侵袭素与细胞表面β 1整合素的相互作用介导。
Quantitative analysis of Yersinia pseudotuberculosis infection of murine gut loops revealed that significantly more wild-type bacteria associated with Peyer's patch M cells than with dome enterocytes or goblet cells. An invasin-deficient mutant was significantly attenuated for M-cell invasion, while beta 1 integrin expression was demonstrated in the apical membranes of M cells but not enterocytes. M-cell targeting by Yersinia pseudotuberculosis in vivo may, therefore, be mediated primarily by the interaction of invasin with cell surface beta 1 integrins.