Genome aware CRISPR gRNA target prediction for parasitic nematodes
Genome aware CRISPR gRNA target prediction for parasitic nematodes
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DOI：
10.1016/j.molbiopara.2018.12.001
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发表时间：
2019-01-01
影响因子：
1.5
通讯作者：
O'Halloran, Damien M.
中科院分区：
文献类型：
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作者：
O'Halloran, Damien M.
最近，为了了解寄生线虫独特的分子生物学而建立遗传模型的研究步伐有所加快。这项研究为各种寄生线虫开发了一套多样化的遗传工具。 CRISPR/Cas9 技术尤其为研究寄生线虫的研究人员提供了作为改变游戏规则的工具的广阔前景。与 RNAi 不同，RNAi 依赖于不同的线虫效应子来沉默基因表达，而 CRISPR/Cas9 突变的效应子通常由实验者提供，这使得通过 CRISPR/Cas9 进行基因编辑成为测试遗传难治性线虫系统的理想选择。为了促进寄生线虫 CRISPR/Cas9 技术的开发，我在这里描述了一种用于识别 gRNA 靶标和针对用户提供的序列的诊断引物的工具。该软件试图通过询问寄生线虫的基因组来最大限度地减少非特异性目标。该软件可在线免费获取，具有直观的界面，可帮助研究人员为寄生线虫设计有效的 CRISPR 实验。
The pace of research towards a genetic model to understand the unique molecular biology of parasitic nematodes has increased recently. This research has developed a diverse suite of genetic tools for a variety of parasitic nematodes. CRISPR/Cas9 technology in particular offers much promise as a game changing tool for researchers studying parasitic nematodes. Unlike RNAi, which depends on diverse nematode effectors to silence gene expression, the effectors for CRISPR/Cas9 mutations are typically supplied by the experimenter, making gene editing via CRISPR/Cas9 ideal for testing on genetically intractable nematode systems. To facilitate the development of CRISPR/Cas9 technology for parasitic nematodes, I here describe a tool for identifying gRNA targets and diagnostic primers to a user supplied sequence. The software attempts to minimize non-specific targets by interrogating the genomes of parasitic nematodes. This software is freely available online and features an intuitive interface to help researchers design effective CRISPR experiments for parasitic nematodes.