養殖ハマチから分離された Strptococcus 属の新魚病細菌について
養殖ハマチから分離された Strptococcus 属の新魚病細菌について
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关于从养殖黄尾鱼中分离出的一种新的链球菌属鱼病细菌
DOI：
10.2331/suisan.42.1345
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发表时间：
1976
影响因子：
0.4
通讯作者：
功 小松
中科院分区：
文献类型：
--
作者：
理一 楠田;研児 川合;利雄 豊嶋;功 小松
1974年7月下旬至9月，高知县鸟清水湾养殖的黄尾鱼发生了一次流行性流行病。典型症状为双侧眼球突出(图1)，穹隆内侧有瘀点，胸尾鳍充血。内有肠、肝、脾、肾充血、出血。病原菌为革兰氏阳性、无孢子卵圆形细胞，呈短链分布，大小约为1.4×0.7μ(图2)。生长温度为10~45℃，最适温度为20~37℃，最适盐浓度为0%，生长浓度范围为0~6.5%。最适生长pH为7.5左右。该菌对氨、明胶酶、尿素酶、吲哚和硫化氢的产生不敏感，对亚甲蓝、荔枝奶和硝酸盐的还原不敏感。过氧化氢酶、淀粉水解酶、细胞色素氧化酶、酪蛋白消化和PT试验均为阴性。MR试验、VP反应和TTC还原均为阳性。由于在6.5%NaC l、pH 9.6和0.1%亚甲基蓝牛奶中生长，该菌被归入链球菌属III群。该菌在生化特性和对鱼的致病性方面与以前建立的任何链球菌属不同。
During the period from late July through September 1974, an epizootic occurred among cultured yellowtails in Tosashimizu Bay in Kochi Prefecture. The typical symptoms of the disease were bilateral exophthalmos (Fig. 1), petechiae on the inside of the opercula and congestion of the pectoral and caudal fins. Internally there was congestion and haemorrhagia of the intestine, liver, spleen and kidney. Tne causative organism was Gram-positive, nonsporing ovoid cells occurring in short chains and about 1.4×0.7μ (Fig. 2). Growth occurred at 10-45°C and optimum temperature appeared to be 20-37°C. Optimum salt concentration for growth was 0%, and growth occurred at the concentration range of 0-6.5%. Optimum pH for growth was about 7.5. The organism was inert to production of ammonia, gelatinase, urease, indol and hydrosulfide, and to reductions by methylene blue, litmas milk and nitrate. Catalase, starch hydrolysis, cytochrome oxdase, casein digestion and PT test were negative. MR test, VP reaction and TTC reduction were positive. Because of the growth in 6.5% NaCl, at pH 9.6 and in 0.1% methylene blue milk, the organism was placed in the genus Streptococcus, group III. The organism was found to be not identical in its biochemical characteristic and pathogenicity to fish to any formerly established species of the genus Streptococcus.