Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor
Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor
复制标题
DOI：
10.1016/j.snb.2015.06.088
复制
发表时间：
2015-12-31
影响因子：
8.4
通讯作者：
Hytonen, Vesa P.
中科院分区：
文献类型：
--
作者：
Auer, Sanna;Koho, Tiia;Hytonen, Vesa P.
在这里，我们描述了使用生物层干涉生物传感器的快速和灵敏的检测病毒特异性抗体从人血清样品。诺如病毒样颗粒和诺如病毒P-颗粒用于使生物传感器尖端功能化。直接从血清样品中检测抗体具有挑战性，但添加金属螯合剂（DAB）与抗人辣根过氧化物酶标记的抗体组合能够增强血清稀释度高达1：100，000的病毒特异性抗体的检测。生物层干涉法比ELISA更快地提供结果，当使用预功能化传感器时，结果只需10-20分钟。因此，生物层干涉法结合DAB增强提供了一个有吸引力的方法，从复杂的样品基质的生物分子的快速和灵敏的定量。(C)2015爱思唯尔B. V.保留所有权利。
Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20 min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices. (C) 2015 Elsevier B.V. All rights reserved.