Nicotine accelerates diabetes-induced retinal changes.
Nicotine accelerates diabetes-induced retinal changes.
复制标题
DOI：
10.3109/02713683.2014.924147
复制
发表时间：
2015-04
影响因子：
2
通讯作者：
Motamedi M
中科院分区：
文献类型：
--
作者：
Boretsky A;Gupta P;Tirgan N;Liu R;Godley BF;Zhang W;Tilton RG;Motamedi M
在已建立的糖尿病啮齿动物模型上，研究尼古丁对糖尿病早期视网膜改变的影响。使用共聚焦扫描激光眼底镜和光谱域光学相干断层扫描相结合的方法对SpragueDawley大鼠进行了检查，以确定尼古丁暴露、糖尿病以及尼古丁和糖尿病的联合影响下视网膜结构的变化。单次注射65 mg/kg链脲佐菌素诱发糖尿病，每日皮下注射尼古丁。根据以视盘为中心的SD-OCT容积扫描(20°×20°)，确定上、下、鼻和颞象限的视网膜厚度。在SD-OCT横切面的子集上进行离散视网膜层的分割，以进一步检查每个治疗组的变化。用共聚焦形态计量学成像评估神经节细胞层(GCL)内神经元的存活情况。对照组在整个研究过程中没有经历任何显著的变化。尼古丁治疗组视网膜总厚度平均减少9.4μm，经分段分析，大部分丢失位于外核层(ONL)。糖尿病组表现出TRT降低的趋势，而DR组的节段分析显示ONL内明显变薄(P<；0.05)。在尼古丁和糖尿病的联合作用下，TRT的μm显著增加(P<0.05)，并伴有GCL神经元数量的减少。在尼古丁暴露、糖尿病以及尼古丁和糖尿病的联合作用下，我们发现视网膜形态发生了显著的时间变化。这些发现可能对糖尿病患者使用含有尼古丁的产品，如香烟、无烟烟草、电子烟或戒烟产品的治疗策略有影响。
To investigate the effects of nicotine on retinal alterations in early stage diabetes in an established rodent model. Sprague-Dawley rats were examined using a combination of confocal scanning laser ophthalmoscopy and spectral domain optical coherence tomography to determine changes in retinal structure in response to nicotine exposure, diabetes and the combined effects of nicotine and diabetes. Diabetes was induced by a single injection of 65 mg/kg streptozotocin and nicotine injections were administered subcutaneously daily. Retinal thickness in the superior, inferior, nasal and temporal quadrants were determined based on SD-OCT volume scans (20° × 20°) centered on the optic disc. Segmentation of discrete retinal layers was performed on a subset of SD-OCT cross-sections to further examine changes in each treatment group. Survival of neurons within the ganglion cell layer (GCL) was assessed by confocal morphometric imaging. The control group did not experience any significant change throughout the study. The nicotine treatment group experienced an average decrease in total retinal thickness (TRT) of 9.4 μm with the majority of the loss localized within the outer nuclear layer (ONL) as determined by segmentation analysis (P < 0.05). The diabetic group exhibited a trend toward decreased TRT while segmentation analysis of the DR group revealed significant thinning within the ONL (P < 0.05). The combination of nicotine and diabetes revealed a significant increase of 8.9 μm in the TRT (P < 0.05) accompanied by a decrease in the number of GCL neurons. We demonstrated significant temporal changes in retinal morphology in response to nicotine exposure, diabetes and with the combined effects of nicotine and diabetes. These findings may have implications in determining treatment strategies for diabetic patients using products containing nicotine such as cigarettes, smokeless tobacco, electronic cigarettes, or smoking cessation products.
登录
查看更多内容
影响因子：
4.4
作者：
Huber G;Beck SC;Grimm C;Sahaboglu-Tekgoz A;Paquet-Durand F;Wenzel A;Humphries P;Redmond TM;Seeliger MW;Fischer MD
通讯作者：
Fischer MD
影响因子：
15.9
作者：
Barber, AJ;Lieth, E;Gardner, TW
通讯作者：
Gardner, TW
影响因子：
8.2
作者：
Karamanos, B;Porta, M;Fuller, JH
通讯作者：
Fuller, JH
影响因子：
4.4
作者：
Bearse, MA;Han, Y;Adams, AJ
通讯作者：
Adams, AJ
DOI：
10.1152/ajprenal.00293.2010
发表时间：
2010-10-01
影响因子：
4.2
作者：
Hua, Ping;Feng, Wenguang;Jaimes, Edgar A.
通讯作者：
Jaimes, Edgar A.