Primary human marrow stromal cells and Saos-2 osteosarcoma cells use different mechanisms to adhere to hydroxylapatite
Primary human marrow stromal cells and Saos-2 osteosarcoma cells use different mechanisms to adhere to hydroxylapatite
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DOI：
10.1002/jbm.a.20043
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发表时间：
2004-02-01
影响因子：
4.9
通讯作者：
Bellis, SL
中科院分区：
文献类型：
--
作者：
Kilpadi, KL;Sawyer, AA;Bellis, SL
在硬组织种植体上形成骨的一个重要步骤是成骨细胞前体与种植体表面的黏附。在这项研究中，我们使用针对整合素亚单位的功能阻断抗体来表征人骨髓基质细胞和Saos-2骨肉瘤细胞黏附于蛋白涂层羟基磷灰石(HA)的机制。我们发现，SAOS-2同时使用包含α(5)和α(V)的整合素，而基质细胞使用包含α(V)的整合素，而不使用包含α(5)的整合素，尽管细胞表面存在包含α(5)的整合素。在这种差异的基础上，我们检测了这些细胞类型与包裹有纤维连接蛋白(FN)或玻璃体连接蛋白(VN)的HA的结合，以确定这些α(5)和α(V)整合素的配体是否能够增加附着在它们上面的细胞的数量或形态。我们还检测了细胞与涂有RGD多肽的HA的粘附性，RGD多肽旨在结合FN或VN受体。与IN或VN相比，血清涂层表面的贴壁基质细胞的形态和数量显著增加，而令人惊讶的是，Saos-2细胞未能在血清涂层的HA上铺展，而在VN涂层的HA上显示出更好的铺展和应力纤维形成。总而言之，这些结果对设计蛋白质涂层以增强HA植入物的性能具有重要意义。(C)2003年威利期刊公司。
One important step in bone formation on hard tissue implants is adhesion of osteoblast precursors to the implant surface. In this study, we used function-blocking antibodies against integrin subunits to characterize the mechanisms used by human marrow stromal cells and Saos-2 osteosarcoma cells to adhere to protein-coated hydroxylapatite (HA). We found that Saos-2 use both alpha(5)- and alpha(v)-containing integrins, whereas stromal cells use alpha(v)-containing integrins but not a.-containing integrins, despite the presence of alpha(5)-containing integrins on cell surfaces. On the basis of this difference, we examined binding of these cell types to HA coated with fibronectin (FN) or vitronectin (VN), to determine whether these ligands for alpha(5) and alpha(v) integrins could enhance the numbers or morphology of cells adhered to them. We also examined the adhesion of cells to HA coated with RGD peptides designed to bind to FN or VN receptors. Morphology and number of adherent stromal cells were markedly enhanced on serum-coated surfaces compared with IN or VN alone, whereas, surprisingly, Saos-2 cells failed to spread on serum-coated HA and displayed superior spreading and stress fiber formation,on VN-coated HA. Collectively, these results have important implications for the design of protein coatings to enhance the performance of HA implants. (C) 2003 Wiley Periodicals, Inc.