Lycorine possesses notable anticancer potentials in on-small cell lung carcinoma cells via blocking Wnt/ b -catenin signaling and epithelial- mesenchymal transition (EMT)
Lycorine possesses notable anticancer potentials in on-small cell lung carcinoma cells via blocking Wnt/ b -catenin signaling and epithelial- mesenchymal transition (EMT)
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Lycorine 通过阻断 Wnt/b-catenin 信号传导和上皮间质转化 (EMT) 在小细胞肺癌细胞中具有显着的抗癌潜力
DOI：
10.1016/j.bbrc.2017.11.032
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发表时间：
2017
影响因子：
3.1
通讯作者：
Zongsuo Liang
中科院分区：
文献类型：
--
作者：
Yanfang Sun;Pingping Wu;Yang Sun;Farukh S. Sharopov;Qi Yang;Fangming Chen;Pan Wang;Zongsuo Liang
石蒜碱是一种天然的异喹啉类生物碱，据报道具有多种抗癌活性。然而，到目前为止，石蒜碱在非小细胞肺癌（NSCLC）中的抗癌作用尚未完全阐明。本文主要研究了石蒜碱抗肿瘤的分子机制。体外和体内非小细胞肺癌。结果表明，石蒜碱明显抑制A549和H460细胞的增殖，IC50值分别为10.83±1.14 mM和12.35±1.13 mM，对正常肺上皮细胞Beas-2B细胞有轻微的细胞毒性，使细胞周期阻滞在G0/G1期。赫斯特dna结合蛋白。染色显示典型的核形态凋亡特征，AnnexinV-FITC/PI染色显示石蒜碱处理后早期凋亡和线粒体膜电位（Djm）耗散。此外，石蒜碱可有效抑制Wnt/b-catenin信号传导并逆转上皮-间质转化（EMT）。此外，石蒜碱还干预了caspase介导的线粒体凋亡途径。此外，A549/Luc肿瘤异种移植模型也证实了石蒜碱显著抑制肺肿瘤的生长和转移，这些数据突出了石蒜碱作为潜在的抗肿瘤药物对抗NSCLC的意义。
Lycorine, an natural isoquinoline alkaloid has reportedly that possesses multi-anticancer activity. However, to date, the anticancer feature of lycorine in non-small cell lung carcinoma (NSCLC) has still not fully been spelled out. The present study mainly focused on the molecular mechanism of lycorine against.NSCLC in vitro and vivo. The results showed that lycorine evidently inhibited proliferation of A549 and H460 with IC50 values were 10.83 ± 1.14 mM and 12.35 ± 1.13 mM, while caused slight cytotoxicity in normal pulmonary epithelial Beas-2B cells, and arrested cell cycle in G0/G1 phase. Hoechst DNA-binding.staining showed that typical characteristics of nuclear morphology apoptosis, AnnexinV-FITC/PI staining revealed the early-period apoptosis and the dissipation of mitochondrial membrane potential (Djm) were also captured after lycorine treatment. Moreover, lycorine effectively repressed the Wnt/b-catenin signaling and reversed epithelial-mesenchymal transition (EMT). In addition, lycorine also intervened the caspase-mediated mitochondrial apoptosis pathway. Furthermore, A549/Luc tumor xenograft model was also corroborated that lycorine significantly suppressed the growth and metastasis of the lung tumor..These data highlight the significance of lycorine as potential anti-neoplastic agents to combat NSCLC.