Linking transcriptomes with morphological and functional phenotypes in mammalian retinal ganglion cells
Linking transcriptomes with morphological and functional phenotypes in mammalian retinal ganglion cells
复制标题
将转录组与哺乳动物视网膜神经节细胞的形态和功能表型联系起来
DOI：
10.1016/j.celrep.2022.111322
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发表时间：
2022-09-13
期刊：
影响因子：
8.8
通讯作者：
Liu, Sheng
中科院分区：
文献类型：
--
作者：
Huang, Wanjing;Xu, Qiang;Liu, Sheng
视网膜神经节细胞（RGC）是大脑通往视觉世界的门户。它们可以根据其电生理学、转录组学或形态学特征分为不同类型。在这里，我们使用Patch测序（Patch-seq）表征了472个高质量RGC的转录组学，形态学和功能特征，为先前建立的RGC图谱的许多转录组学定义的细胞类型提供了功能和形态学注释。我们展示了不同模态在定义RGC身份方面的收敛，并揭示了多模态数据中充分表征的细胞类型的对应程度。此外，我们补充了一些RGC类型与详细的形态和功能特性。我们还确定了ON，OFF和ON-OFF RGCs之间的差异表达基因，如Vat 11，Slitrk 6和Lmo 7，为功能研究提供候选标记基因。我们的研究表明，分子上不同的簇在编码视觉信息的作用上也可能不同。
Retinal ganglion cells (RGCs) are the brain's gateway to the visual world. They can be classified into different types on the basis of their electrophysiological, transcriptomic, or morphological characteristics. Here, we characterize the transcriptomic, morphological, and functional features of 472 high-quality RGCs using Patch sequencing (Patch-seq), providing functional and morphological annotation of many transcriptomic-defined cell types of a previously established RGC atlas. We show a convergence of different modalities in defining the RGC identity and reveal the degree of correspondence for well-characterized cell types across multi -modal data. Moreover, we complement some RGC types with detailed morphological and functional proper-ties. We also identify differentially expressed genes among ON, OFF, and ON-OFF RGCs such as Vat1l, Slitrk6, and Lmo7, providing candidate marker genes for functional studies. Our research suggests that the molecularly distinct clusters may also differ in their roles of encoding visual information.