Co-evolved plant and blast fungus ascorbate oxidases orchestrate the redox state of host apoplast to modulate rice immunity
Co-evolved plant and blast fungus ascorbate oxidases orchestrate the redox state of host apoplast to modulate rice immunity
复制标题
共同进化的植物和稻瘟菌抗坏血酸氧化酶协调宿主质外体的氧化还原状态来调节水稻免疫力
DOI：
10.1016/j.molp.2022.07.001
复制
发表时间：
2022-08-04
期刊：
影响因子：
27.5
通讯作者：
Zhang, Zhengguang
中科院分区：
文献类型：
--
作者：
Hu, Jiexiong;Liu, Muxing;Zhang, Zhengguang
外质体抗坏血酸氧化酶（AOs）通过调节外质体氧化还原状态，在活性氧（ROS）介导的先天宿主免疫中起关键作用。迄今为止，关于稻瘟病菌Magnaporthe oryzae的外体效应因子如何调节水稻外体氧化还原状态从而破坏植物免疫，我们知之甚少。在这项研究中，我们证明了m.o ryzae MoAo1是一种通过调节水稻细胞外质体氧化还原状态而起毒力作用的AO。我们发现MoAo1抑制水稻OsAO3和OsAO4的活性，而OsAO3和OsAO4也调节外质体氧化还原状态和植物免疫。此外，我们发现MoAo1、OsAO3和OsAO4都表现出多态性变异，其不同的相互作用协调了病原体的毒力和水稻的免疫。综上所述，我们的研究结果揭示了细胞外氧化还原酶在稻瘟病侵染过程中的关键作用，并揭示了外质体氧化还原状态及其调控在植物与病原体相互作用中的重要性。
Apoplastic ascorbate oxidases (AOs) play a critical role in reactive oxygen species (ROS)-mediated innate host immunity by regulating the apoplast redox state. To date, little is known about how apoplastic effec-tors of the rice blast fungus Magnaporthe oryzae modulate the apoplast redox state of rice to subvert plant immunity. In this study, we demonstrated that M. oryzae MoAo1 is an AO that plays a role in virulence by modulating the apoplast redox status of rice cells. We showed that MoAo1 inhibits the activity of rice OsAO3 and OsAO4, which also regulate the apoplast redox status and plant immunity. In addition, we found that MoAo1, OsAO3, and OsAO4 all exhibit polymorphic variations whose varied interactions orchestrate pathogen virulence and rice immunity. Taken together, our results reveal a critical role for extracellular redox enzymes during rice blast infection and shed light on the importance of the apoplast redox state and its regulation in plant-pathogen interactions.