Detecting apoptosis using dynamic light scattering with optical coherence tomography
Detecting apoptosis using dynamic light scattering with optical coherence tomography
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DOI：
10.1117/1.3600770
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发表时间：
2011-07-01
影响因子：
3.5
通讯作者：
Kolios, Michael C.
中科院分区：
文献类型：
--
作者：
Farhat, Golnaz;Mariampillai, Adrian;Kolios, Michael C.
使用光学相干断层扫描（OCT）实现动态光散射技术以测量细胞经历凋亡时细胞内运动的变化。用顺铂处理急性髓性白血病细胞，并在48小时内的不同时间使用1300 nm扫频源OCT系统以166 Hz的帧速率成像。斑点强度的时间相关性分析表明治疗后24小时细胞内运动显著增加。这种细胞内运动的增加与不规则形状和破碎细胞的组织学发现相关，表明细胞膜起泡和破碎。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI 10.1117/1.3600770]
A dynamic light scattering technique is implemented using optical coherence tomography (OCT) to measure the change in intracellular motion as cells undergo apoptosis. Acute myeloid leukemia cells were treated with cisplatin and imaged at a frame rate of 166 Hz using a 1300 nm swept-source OCT system at various times over a period of 48 h. Time correlation analysis of the speckle intensities indicated a significant increase in intracellular motion 24 h after treatment. This rise in intracellular motion correlated with histological findings of irregularly shaped and fragmented cells indicative of cell membrane blebbing and fragmentation. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3600770]