Phytol supplementation alters plasma concentrations of formate, amino acids, and lipid metabolites in sheep
Phytol supplementation alters plasma concentrations of formate, amino acids, and lipid metabolites in sheep
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DOI：
10.1016/j.animal.2021.100174
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发表时间：
2021-03-27
期刊：
影响因子：
3.6
通讯作者：
Sugino，T.
中科院分区：
文献类型：
--
作者：
Ding，L. L.;Matsumura，M.;Sugino，T.
叶绿素分子中的植醇部分在单胃动物中作为过氧化物酶体增殖物激活受体-α的激动剂。本研究旨在阐明绵羊日粮中添加植物醇对血浆甲酸盐和与一碳（1C）供体相关的氨基酸浓度的影响及其对脂质代谢的影响。用大麦、米糠、豆粕和燕麦干草组成的混合日粮（代谢能10.7 MJ/kg DM;粗蛋白150 g/kg DM）以维持代谢能的1.5倍饲喂4只成年绵羊，连续3个14 d试验期。第一和第三阶段作为对照组，不添加叶绿醇，而在第二阶段，叶绿醇添加到混合日粮中，每天12克/公斤的膳食DM。在每个阶段，采集粪便、尿液和颈静脉血样。干物质采食量与代谢体重的关系在第一阶段略低于第二和第三阶段（P< 0.01），但在后两个阶段之间没有差异。干物质消化率略有降低（P= 0.05）的植醇治疗。氮（N）的摄入量和保留显示出类似的趋势，DM摄入量，但尿氮是没有变化的时期。叶绿醇处理期间血浆胆固醇和磷脂浓度降低，而甘油三酯浓度升高（P< 0.05）。在植醇处理期间，血浆丝氨酸和甘氨酸（1C供体）浓度升高，而谷氨酸水平降低（P< 0.01）。从第一个对照期到植醇补充期，甲酸盐和蛋氨酸的血浆浓度增加（P< 0.01），但同型半胱氨酸和半胱氨酸（蛋氨酸循环的中间产物和副产物）水平在处理期之间没有变化。总之，植物醇影响绵羊的脂肪代谢以及氨基酸代谢和1C供体。这些影响可能与植醇作为核受体激动剂的活性有关，但这需要进一步研究。
The phytol moiety in chlorophyll molecules acts as an agonist of peroxisome proliferator-activated receptor-α in monogastric animals. The current study aimed to clarify the effects of dietary supplementation with phytol on the plasma concentrations of formate and amino acids related to one-carbon (1C) donors and its effects on lipid metabolism in sheep. Four mature sheep were fed with a mixed ration (metabolizable energy, 10.7 MJ/kg DM; CP, 150 g/kg DM) comprising barley, rice bran, soybean meal, and oat hay at 1.5 times maintenance metabolizable energy for three consecutive 14-day experimental periods. The first and third periods served as controls without phytol supplementation, while in the second period, phytol was added to the mixed ration at 12 g/kg of dietary DM per day. In each period, feces, urine, and jugular blood samples were collected. Dry matter intake in relation to metabolic BW was slightly lower (P< 0.01) in the first period than the second and third periods but did not differ between the latter two periods. Dry matter digestibility was slightly reduced (P= 0.05) by the phytol treatment. Nitrogen (N) intake and retention showed similar trends to DM intake, but urinary N was unchanged among the periods. Plasma cholesterol and phospholipid concentrations decreased during the phytol treatment period, while triglyceride concentration increased (P< 0.05). In the phytol treatment period, the plasma concentrations of serine and glycine (1C donors) increased, but the glutamate level decreased (P< 0.01). Plasma concentrations of formate and methionine increased (P< 0.01) from the first control period to the phytol supplementation period, but homocysteine and cysteine (intermediate and by-product of the methionine cycle) levels were unchanged among the treatment periods. In conclusion, dietary phytol affects lipid metabolism as well as amino acid metabolism and 1C donors in sheep. These effects may be associated with the activity of phytol as an agonist of the nuclear receptors, although this needs further investigation.