Replication protein A safeguards genome integrity by controlling NER incision events.
Replication protein A safeguards genome integrity by controlling NER incision events.
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DOI：
10.1083/jcb.201006011
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发表时间：
2011-02-07
期刊：
影响因子：
--
通讯作者：
Fousteri M
中科院分区：
文献类型：
--
作者：
Overmeer RM;Moser J;Volker M;Kool H;Tomkinson AE;van Zeeland AA;Mullenders LH;Fousteri M
Continued association of RPA with sites of incomplete nucleotide excision repair averts further incision events until repair is completed. Single-stranded DNA gaps that might arise by futile repair processes can lead to mutagenic events and challenge genome integrity. Nucleotide excision repair (NER) is an evolutionarily conserved repair mechanism, essential for removal of helix-distorting DNA lesions. In the currently prevailing model, NER operates through coordinated assembly of repair factors into pre- and post-incision complexes; however, its regulation in vivo is poorly understood. Notably, the transition from dual incision to repair synthesis should be rigidly synchronized as it might lead to accumulation of unprocessed repair intermediates. We monitored NER regulatory events in vivo using sequential UV irradiations. Under conditions that allow incision yet prevent completion of repair synthesis or ligation, preincision factors can reassociate with new damage sites. In contrast, replication protein A remains at the incomplete NER sites and regulates a feedback loop from completion of DNA repair synthesis to subsequent damage recognition, independently of ATR signaling. Our data reveal an important function for replication protein A in averting further generation of DNA strand breaks that could lead to mutagenic and recombinogenic events.
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DOI：
10.1073/pnas.0603779103
发表时间：
2006-06-27
影响因子：
11.1
作者：
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通讯作者：
Cleaver, James E.
影响因子：
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作者：
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影响因子：
16
作者：
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通讯作者：
Mullenders, Leon H. F.
影响因子：
5.3
作者：
Essers, J;Theil, AF;Vermeulen, W
通讯作者：
Vermeulen, W
DOI：
10.1083/jcb.200902150
发表时间：
2009-09-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Marteijn JA;Bekker-Jensen S;Mailand N;Lans H;Schwertman P;Gourdin AM;Dantuma NP;Lukas J;Vermeulen W
通讯作者：
Vermeulen W