Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy
Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy
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DOI：
10.1016/s1044-0305(97)00060-3
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发表时间：
1997-04-01
影响因子：
3.2
通讯作者：
McLafferty, FW
中科院分区：
文献类型：
--
作者：
Kelleher, NL;Senko, MW;McLafferty, FW
以前，产生单位分辨率质谱的样品的最高分子量为67 kDa（边缘为86 kDa），采用6.2 T傅里叶变换质谱仪电喷雾电离获得。现在使用9.4 T仪器，软骨素酶I（997个氨基酸）和II（990个氨基酸）的分辨率已达到170，000，可能的分子量分别为112，509和111，714，而计算的分子量为112，508和111，713。协助这些分配的是通过Senko、马歇尔及其同事介绍的时域数据采样技术实现的分辨同位素峰的噪声降低。(C)1997年美国质谱学会。
Previously, the highest molecular weight for a sample yielding a unit resolution mass spectrum was 67 kDa (marginal at 86 kDa), obtained with a 6.2 T Fourier-transform mass spectrometer with electrospray ionization. Now with a 9.4 T instrument, resolving power of 170,000 has been achieved for chondroitinase I (997 amino acids) and II (990 amino acids), making possible molecular weight assignments of 112,509 and 111,714, respectively, versus 112,508 and 111,713 calculated. Assisting these assignments was the noise reduction in the resolved isotopic peaks achieved by the time domain data sampling technique introduced by Senko, Marshall, and co-workers. (C) 1997 American Society for Mass Spectrometry.