The mouse Pdgfc gene:: dynamic expression in embryonic tissues during organogenesis
The mouse Pdgfc gene:: dynamic expression in embryonic tissues during organogenesis
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DOI：
10.1016/s0925-4773(00)00425-1
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发表时间：
2000-09-01
影响因子：
2.6
通讯作者：
Nagy, A
中科院分区：
文献类型：
--
作者：
Ding, H;Wu, XL;Nagy, A
血小板源性生长因子 A 和 B（PDCF-A 和 PDGF-B）的信号传导活性通过两种受体激酶 PDGFR-α 和 PDGFR-β 介导，已被证明对于心血管器官、肾脏、肺和中枢神经系统的发育至关重要。在克隆 VEGF 相关蛋白基因的过程中，我们分离出了可以编码 345 个氨基酸的蛋白的小鼠 cDNA。氨基酸序列的比较表明，该预测的基因产物与人 PDGF-C 有 95% 的同一性。小鼠 Pdgfc 基因映射到与人类染色体 6p21.3 同线的 17 号染色体区域。在 E9.5-E15.5 小鼠胚胎中，Pdgfc 在表面外胚层中广泛表达，随后在皮肤生发层、嗅觉和耳基板及其衍生物以及口腔内壁中表达。在肠道和内脏器官中，例如肺和肾，Pdgfc mRNA首先在内胚层上皮中表达，然后在与内胚层结构相关的间充质组织中表达。与其他 PDGF 类似，Pdgfc 广泛表达于间充质前体以及平滑肌和骨骼肌的成肌细胞中。与PDGF-A相反，除小脑和神经嵴细胞的神经源性衍生物外，Pdgfc不在中枢神经系统中表达。 Pdgfc 也是关于心脏和血管内皮 (C) 2000 Elsevier Science ireland Ltd. 保留所有权利。
The signaling activity of Platelet-derived growth factors A and B (PDCF-A and PDGF-B) that is mediated through the two receptor kinases, PDGFR-alpha and PDGFR-beta has been shown to be critical for the development of the cardiovascular organs, the kidney, the lung and the central nervous system. During the cloning of genes for VEGF related proteins, we isolated a mouse cDNA that can encode for a protein of 345 amino acids. A comparison of the amino acid sequence reveals that this predicted gene product displays 95% identity to human PDGF-C. The mouse Pdgfc gene maps to a region of chromosome 17 that is syntenic to human chromosome 6p21.3 In E9.5-E15.5 mouse embryo, Pdgfc is widely expressed in the surface ectoderm and later in the germinal layer of the skin, the olfactory and otic placode and their derivatives and the lining of the oral cavity. In the gut and visceral organs, such as the lung and the kidney, Pdgfc mRNA is first expressed in the endodermal epithelium and later in mesenchymal tissues associated with the endodermal structures. Similar to other PDGFs, Pdgfc is widely expressed in mesenchymal precursors and the myoblast of the smooth and skeletal muscles. Contrary to PDGF-A, Pdgfc is not expressed in the central nervous system, except in the cerebellum and neurogenic derivatives of the neural crest cells. Pdgfc is also about from the heart and the vascular endothelium (C) 2000 Elsevier Science ireland Ltd. All rights reserved.