Timed changes of synaptic zinc, synaptophysin and MAP2 in medial extended amygdala of epileptic animals are suggestive of reactive neuroplasticity
Timed changes of synaptic zinc, synaptophysin and MAP2 in medial extended amygdala of epileptic animals are suggestive of reactive neuroplasticity
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DOI：
10.1016/j.brainres.2010.01.087
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发表时间：
2010-04-30
期刊：
影响因子：
2.9
通讯作者：
Beltramino, Carlos A.
中科院分区：
文献类型：
--
作者：
Pereno, German I.;Beltramino, Carlos A.
反复癫痫发作在实验模型和难治性颞叶癫痫（TLE）患者中诱导大脑的永久性改变，TLE是人类常见的癫痫形式。与许多脑区域中的细胞损失和神经胶质增生一起，主要在海马中观察到突触重组。然而，在杏仁核中，这种突触重组尚未被研究。本研究以海人酸（KA）诱发颞叶癫痫模型，观察了杏仁核内侧延伸区Zn浓度、突触素和MAP（2）的变化。在KA腹腔注射（9 mg/kg）后10天、1、2、3和4个月对成年雄性大鼠（n=6）进行灌注。对照组注射生理盐水。通过Timm方法处理大脑以揭示突触Zn并通过密度测定法进行分析。免疫组化法检测突触素和MAP（2）的表达。采用双因素方差分析进行统计，P
Repeated seizures induce permanent alterations of the brain in experimental models and patients with intractable temporal lobe epilepsy (TLE), which is a common form of epilepsy in humans. Together with cell loss and gliosis in many brain regions, synaptic reorganization is observed principally in the hippocampus. However, in the amygdala this synaptic reorganization has been not studied. The changes in Zn density, synaptophysin and MAP(2) as markers of reactive synaptogenesis in medial extended amygdala induced by kainic acid (KA) as a model of TLE was studied. Adult male rats (n=6) were perfused at 10 days, 1, 2, 3 and 4 months after KA i.p. injection (9 mg/kg). Controls were injected with saline. The brains were processed by the Timm's method to reveal synaptic Zn and analyzed by densitometry. Immunohistochemistry was used to reveal synaptophysin and MAP(2) expression. A two-way ANOVA was used for statistics, with a P