Polyphosphoric acid capping radioactive/upconverting NaLuF4:Yb,Tm,Sm-153 nanoparticles for blood pool imaging in vivo
Polyphosphoric acid capping radioactive/upconverting NaLuF4:Yb,Tm,Sm-153 nanoparticles for blood pool imaging in vivo
复制标题
用于体内血池成像的多磷酸封端放射性/上转换NaLuF4:Yb,Tm,Sm-153纳米粒子
DOI：
10.1016/j.biomaterials.2013.07.098
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发表时间：
2013
期刊：
影响因子：
14
通讯作者：
Li Fuyou
中科院分区：
文献类型：
--
作者：
Peng Juanjuan;Sun Yun;Zhao Lingzhi;Wu Yongquan;Feng Wei;Gao Yanhong;Li Fuyou
在血液中长时间循环的纳米颗粒具有重要的生物医学应用。然而，迄今为止，尚未报道具有长期循环血流的基于镧系元素的纳米粒子的例子。在此，我们报道了涂有多磷酸配体（乙二胺四亚甲基膦酸（EDTMP））的双功能放射性和上转换纳米颗粒（UCNP），用于单光子发射计算机断层扫描（SPECT）血池成像。使用透射电子显微镜和动态光散射验证了 EDTMP 涂层纳米颗粒 (EDTMP-UCNP) 的结构、尺寸和 zeta 电位。将放射性同位素钐-153 标记的 EDTMP-UCNP (EDTMP-UCNP:153Sm) 注射到小鼠体内显示出与涂有柠檬酸 (cit-UCNP:153Sm) 和 EDTMP 153Sm 复合物 (EDTMP-153Sm) 的对照纳米颗粒相比，其循环时间更佳。延长循环时间的机制可能归因于 EDTMP-UCNP 在红细胞 (RBC) 膜上的粘附。体内毒性结果显示，EDTMP-UCNP 在 100 mg/kg 剂量下没有毒性，验证了其作为血池成像剂的安全性。我们的结果通过引入多磷酸作为表面配体，为长期循环血流提供了纳米探针的新策略。
Nanoparticles that circulate in the bloodstream for a prolonged period of time have important biomedicine applications. However, no example of lanthanide-based nanoparticles having a long-term circulation bloodstream has been reported to date. Herein, we report on difunctional radioactive and upconversion nanoparticles (UCNP) coated with polyphosphoric acid ligand, that is ethylenediamine tetramethylenephosphonic acid (EDTMP), for an application in single-photon emission computed tomography (SPECT) blood pool imaging. The structure, size and zeta-potential of the EDTMP-coated nanoparticles (EDTMP-UCNP) are verified using transmission electron microscopy and dynamic light scattering. Injection of radioisotope samarium-153-labeled EDTMP-UCNP (EDTMP-UCNP:153Sm) into mice reveal superior circulation time compared to control nanoparticles coated with citric acid (cit-UCNP:153Sm) and153Sm complex of EDTMP (EDTMP-153Sm). The mechanism for the extended circulation time may be attributed to the adhesion of EDTMP-UCNP on the membrane of red blood cells (RBCs).In vivotoxicity results show no toxicity of EDTMP-UCNP at the dose of 100 mg/kg, validating its safety as an agent for blood pool imaging. Our results provide a new strategy of nanoprobe for a long-term circulation bloodstream by introducing polyphosphoric acid as surface ligand.