Vascular wall resident progenitor cells:: a source for postnatal vasculogenesis
Vascular wall resident progenitor cells:: a source for postnatal vasculogenesis
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DOI：
10.1242/dev.02315
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发表时间：
2006-04-15
期刊：
影响因子：
4.6
通讯作者：
Erg端n, S
中科院分区：
文献类型：
--
作者：
Zengin, E;Chalajour, F;Erg端n, S
在这里，我们报道了内皮前体（EPC）和干细胞在血管壁的不同区域的存在，它们能够分化为成熟的内皮细胞、造血细胞和局部免疫细胞，如巨噬细胞。该区域已被确定为定位于平滑肌和成人血管壁外皮层之间。它主要含有cd34阳性（+）和cd31阴性（-）细胞，这些细胞也表达VEGFR2和TIE2。在血管壁的这个区域只有少数细胞CD45阳性。在使用人胸内动脉（HITA）片段的环状实验中，我们发现HITA壁的CD34+细胞在体外形成毛细血管芽，并且显然被肿瘤细胞招募用于毛细血管形成。这些血管壁上的内皮细胞形成的新血管表达血管生成激活的内皮细胞的标志物，如CEACAM1，也表达成熟内皮细胞的标志物，如VE-cadherin或occludin。在研究的所有器官的大、中型动脉和静脉中都发现了含有内皮祖细胞的血管壁区域。这些数据表明，在成人血管壁上存在“血管生成区”，这可能是出生后血管生成的祖细胞的来源，有助于肿瘤血管化和局部免疫反应。
Here, we report the existence of endothelial precursor ( EPC) and stem cells in a distinct zone of the vascular wall that are capable to differentiate into mature endothelial cells, hematopoietic and local immune cells, such as macrophages. This zone has been identified to be localized between smooth muscle and adventitial layer of human adult vascular wall. It predominantly contains CD34-positive (+) but CD31-negative (-) cells, which also express VEGFR2 and TIE2. Only few cells in this zone of the vascular wall are positive for CD45. In a ring assay using the fragments of human internal thoracic artery (HITA), we show here that the CD34+ cells of the HITA-wall form capillary sprouts ex vivo and are apparently recruited for capillary formation by tumor cells. New vessels formed by these vascular wall resident EPCs express markers for angiogenically activated endothelial cells, such as CEACAM1, and also for mature endothelial cells, such as VE-cadherin or occludin. Vascular wall areas containing EPCs are found in large and middle sized arteries and veins of all organs studied here. These data suggest the existence of a 'vasculogenic zone' in the wall of adult human blood vessels, which may serve as a source for progenitor cells for postnatal vasculogenesis, contributing to tumor vascularization and local immune response.