Muscarinic M2 stimulation releases histamine in the totally isolated, vascularly perfused rat stomach.
Muscarinic M2 stimulation releases histamine in the totally isolated, vascularly perfused rat stomach.
复制标题
毒蕈碱 M2 刺激会在完全隔离、血管灌注的大鼠胃中释放组胺。
DOI：
10.3109/00365528809090168
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发表时间：
1988
影响因子：
1.9
通讯作者：
Helge L. Waldum
中科院分区：
文献类型：
--
作者：
A. Sandvik;P. M. Kleveland;Helge L. Waldum
本研究在完全隔离、血管灌注的大鼠胃中检验了组胺在迷走神经刺激和毒蕈碱 M1 激动剂 McN-A-343 诱导的酸分泌刺激中的作用。这些刺激与刺激 cAMP 系统的药物（异丁基甲基黄嘌呤 (IMX) 或毛喉素）、毒蕈碱拮抗剂（阿托品或哌仑西平）或组胺 H2 拮抗剂（雷尼替丁）相结合。 IMX和毛喉素增强了McN-A-343刺激的酸分泌，产生的酸输出分别是McN-A-343-和IMX-或McN-A-343-和毛喉素刺激的输出总和的280%和260%。雷尼替丁抑制 McN-A-343 单独或与 IMX 组合刺激的酸分泌，而毛喉素刺激的分泌不受 H2 拮抗剂的影响。这强烈表明内源性组胺通过增加壁细胞 cAMP 来增强毒蕈碱 M1 刺激的酸分泌。用 IMX 刺激迷走神经使酸输出量从 12.2 +/- 3.0 增加到 49.2 +/- 9.3 mumol/60 分钟（平均值 +/- SEM）。 M1 拮抗剂哌仑西平和 M1/M2 拮抗剂阿托品均显着（p 小于 0.01）抑制迷走神经刺激的酸分泌。 McN-A-343 测得的静脉流出物中的组胺输出量没有变化，而神经刺激引起静脉组胺输出量明显增加，从神经刺激前的 101 +/- 21 增加到神经刺激开始后的 212 +/- 28 pmol/min（平均值 +/- SEM）。阿托品将组胺释放降低至基线水平，但哌仑西平仅无显着抑制，表明毒蕈碱 M2 刺激大鼠胃中的组胺释放。
The present study examines the role of histamine in the stimulation of acid secretion induced by vagal nerve stimulation and by the muscarinic M1 agonist McN-A-343 in the totally isolated, vascularly perfused rat stomach. The stimuli were combined with an agent stimulating the cAMP system (isobutyl methylxanthine (IMX) or forskolin), a muscarinic antagonist (atropine or pirenzepine), or a histamine H2 antagonist (ranitidine). IMX and forskolin potentiated McN-A-343-stimulated acid secretion, yielding acid outputs of 280% and 260% of the sum of McN-A-343- and IMX-, or McN-A-343- and forskolin-stimulated outputs, respectively. Ranitidine inhibited acid secretion stimulated by McN-A-343 alone or in combination with IMX, whereas the forskolin-stimulated secretion was not influenced by the H2 antagonist. This strongly indicates that endogenous histamine potentiates muscarinic M1-stimulated acid secretion by increasing parietal cell cAMP. Vagal nerve stimulation with IMX increased acid output from 12.2 +/- 3.0 to 49.2 +/- 9.3 mumol/60 min (mean +/- SEM). The M1 antagonist pirenzepine and the M1/M2 antagonist atropine both significantly (p less than 0.01) inhibited vagally stimulated acid secretion. Histamine output as measured in the venous effluent was unchanged by McN-A-343, whereas nerve stimulation induced a clear increase in venous histamine output, from 101 +/- 21 before to 212 +/- 28 pmol/min (mean +/- SEM) after initiation of nerve stimulation. Histamine release was reduced to base-line levels by atropine but only insignificantly inhibited by pirenzepine, indicating a muscarinic M2 stimulation of histamine release in the rat stomach.
胃刺激剂对分离的犬壁细胞[14 C]氨基比林积累的增强相互作用。
影响因子：
29.4
作者：
Soll,AH
通讯作者：
Soll,AH
分离的犬壁细胞上的胃泌素受体。
DOI：
10.1172/jci111348
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Soll,AH;Amirian,DA;Thomas,LP;Reedy,TJ;Elashoff,JD
通讯作者：
Elashoff,JD