Effects of cyclic tensile forces on the expression of vascular endothelial growth factor (VEGF) and macrophage-colony-stimulating factor (M-CSF) in murine osteoblastic MC3T3-E1 cells
Effects of cyclic tensile forces on the expression of vascular endothelial growth factor (VEGF) and macrophage-colony-stimulating factor (M-CSF) in murine osteoblastic MC3T3-E1 cells
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DOI：
10.1177/154405910508400505
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发表时间：
2005-05-01
影响因子：
7.6
通讯作者：
Tanne, K
中科院分区：
文献类型：
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作者：
Motokawa, M;Kaku, M;Tanne, K
据报道，由成骨细胞表达的血管内皮生长因子（VEGF）可以诱导破骨细胞募集，从而影响骨重塑。本研究的目的是探讨循环张力对成骨细胞MC3T3-E1中VEGF和巨噬细胞集落刺激因子（M-CSF）表达的影响。采用实时荧光定量PCR和酶联免疫分析法检测VEGF和M-CSF基因表达及蛋白浓度。实验组VEGF和M-CSF mRNA的表达高于对照组。实验组VEGF和M-CSF蛋白浓度的升高具有时间依赖性。此外，钆（一种S-A通道抑制剂），而不是硝苯地平（l型Ca2+通道阻滞剂），治疗降低了实验组中VEGF和M-CSF mRNA和蛋白的浓度。这些发现表明，循环拉伸力通过拉伸激活通道（S-A通道）增加成骨细胞MC3T3-E1中VEGF和M-CSF的表达。
It has been reported that vascular endothelial growth factor (VEGF), expressed by osteoblasts, can induce osteoclast recruitment and thus affects bone remodeling. The purpose of this study was to investigate the effects of cyclic tensile forces on the expression of VEGF and macrophage-colony-stimulating factor (M-CSF) in osteoblastic MC3T3-E1 cells. VEGF and M-CSF gene expression and protein concentration were determined by real-time PCR and enzyme-linked immunoassay. The expression of VEGF and M-CSF mRNA in the experimental group was higher than in the control group. The increase in the concentration of VEGF and M-CSF protein in the experimental group was time-dependent. Moreover, gadolinium (an S-A channel inhibitor), but not nifedipine (L-Type Ca2+ channel blocker), treatment reduced the concentration of VEGF and M-CSF mRNA and protein in the experimental groups. These findings suggest that cyclic tensile forces increase the expression of VEGF and M-CSF in osteoblastic MC3T3-E1 cells via a stretch-activated channel (S-A channel).