Performance of a new hybrid artificial liver support system using hepatocytes entrapped within a hydrogel.
Performance of a new hybrid artificial liver support system using hepatocytes entrapped within a hydrogel.
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使用水凝胶内包埋的肝细胞的新型混合人工肝支持系统的性能。
DOI：
10.1097/00002480-198907000-00129
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
N. Ohshima
中科院分区：
文献类型：
--
作者：
K. Yanagi;K. Ookawa;S. Mizuno;N. Ohshima
为了开发一种混合型人工肝支持系统（ALSS），作者利用海藻酸钙水凝胶包裹肝细胞构建了一个旋转圆盘型的ALSS。该模块是模仿气液接触器设计的，使用相同的原理。40个装有薄膜状水凝胶的圆盘安装在水平旋转轴上，与血液保持接触。凝胶中肝细胞的浓度从1.9 × 10(9)到1.7 × 10（10）个细胞/L不等。水凝胶包埋肝细胞使肝细胞保持活力，体外实验结果显示氨代谢和尿素合成。急性肝功能不全猫体外灌注实验结果表明，该模块在体内具有替代肝功能的能力。
To develop a hybrid artificial liver support system (ALSS), the authors constructed a rotating-disk type ALSS using hepatocytes entrapped within a calcium alginate hydrogel. This module was designed in imitation of the gas-liquid contactor that uses the same principle. Forty disks with film-shaped hydrogel were mounted to a horizontal rotating axis and were kept in contact with blood. The concentration of hepatocytes in the gel varied from 1.9 X 10(9) to 1.7 X 10(10) cells/L gel. Entrapping hepatocytes within hydrogel kept the hepatocytes viable, and results of in vitro experiments showed ammonium metabolism and urea synthesis. The results of ex vivo perfusion experiments using cats with acute hepatic insufficiency indicated that this module had the ability to replace liver function in vivo.