THE ETS MULTIGENE FAMILY IS CONSERVED THROUGHOUT THE METAZOA
THE ETS MULTIGENE FAMILY IS CONSERVED THROUGHOUT THE METAZOA
复制标题
DOI：
10.1093/nar/21.15.3479
复制
发表时间：
1993-07-25
影响因子：
14.9
通讯作者：
MORSE, DE
中科院分区：
文献类型：
--
作者：
DEGNAN, BM;DEGNAN, SM;MORSE, DE
这项研究提供了第一个经验证据的保守的ets原癌基因转录因子家族在整个后生动物。使用聚合酶链反应与简并引物对应的ETS DNA结合域内的保守序列，我们已经检测到ets基因在一系列较低的后生动物，包括海绵，栉水母，海葵，扁形虫和线虫，并在几个较高的无脊椎动物后生动物。这些序列中的许多序列与原始的v-ets-1癌基因显著不同，尽管大多数可以与ets基因家族中最近定义的组对齐。多个ETS结构域序列中检测到的一些较低的后生动物物种，提供证据的ETS多基因家族在后生动物进化的最早阶段的存在。与此相反，我们无法检测到任何ETS序列在真菌，植物或几种原生动物的DNA。我们的研究结果表明，负责产生多基因家族的ets原癌基因的复制和分化伴随着后生动物的发育而发生。此外，这些数据证实了其他最近的分子证据，为所有多细胞动物（包括海绵）的单系起源提供了强有力的支持。
This study provides the first empirical evidence for the conservation of the ets proto-oncogene transcription factor family throughout the Metazoa. Using the polymerase chain reaction with degenerate primers corresponding to conserved sequences within the ETS DNA-binding domain, we have detected ets genes in a range of lower metazoans, including sponges, ctenophores, anemones, flatworms and nematodes, and in several higher invertebrate metazoans. Many of these sequences are significantly divergent from the original v-ets-1 oncogene, although most can be aligned with recently defined groups within the ets gene family. Multiple ETS domain sequences were detected in a number of the lower metazoan species, providing evidence for the existence of an ets multigene family at the earliest stages of metazoan evolution. In contrast, we were unable to detect any ETS sequences in fungal, plant or several protozoan DNAs. Our findings suggest that the duplication and divergence of ets proto-oncogenes responsible for generating the multigene family occurred concomitantly with the development of metazoan animals. In addition, these data corroborate other recent molecular evidence in providing strong support for the monophyletic origin of all multicellular animals, including sponges.