Low‐scale syringe‐made synthesis of 15 N‐labeled oligonucleotides
Low‐scale syringe‐made synthesis of 15 N‐labeled oligonucleotides
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低规模注射器合成 15 个 N 标记寡核苷酸
DOI：
10.1002/jlcr.4000
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发表时间：
2022
影响因子：
1.8
通讯作者：
Marek, Aleš
中科院分区：
文献类型：
--
作者：
Brož, Břetislav;Tureček, František;Marek, Aleš
传达了代表DNA三核苷酸密码子的15N标记寡核苷酸的快速且合理的低规模（200nmol）注射器合成。所有密码子均通过固相控制孔玻璃合成柱技术通过亚磷酰胺法制备。使用市售试剂和经济实惠的设备在相当短的时间内制备了涵盖 DNA 遗传密码字母表的 24 种标记寡核苷酸，其产率和纯度可以接受，可直接应用于质谱分析。
Fast and reasonable low‐scale (200 nmol) syringe‐made synthesis of15N‐labeled oligonucleotides representing DNA trinucleotide codons is communicated. All codons were prepared by solid‐phase controlled pore glass synthesis column technique via the phosphoramidite method. Twenty‐four labeled oligonucleotides covering the DNA genetic code alphabet were prepared using commercially available reagents and affordable equipment in a reasonably short period of time, with acceptable yields and purity for direct applications in mass spectrometry.