MOLECULAR-BASIS OF VALINE RESISTANCE IN ESCHERICHIA-COLI K-12
MOLECULAR-BASIS OF VALINE RESISTANCE IN ESCHERICHIA-COLI K-12
复制标题
DOI：
10.1073/pnas.78.2.922
复制
发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
HATFIELD, GW
中科院分区：
文献类型：
--
作者：
LAWTHER, RP;CALHOUN, DH;HATFIELD, GW
确定了valine抗性与E.coliK-12的ilvGEDA操纵子表达的关系。DNA序列和体内蛋白质分析表明，在野生型大肠杆菌K-12中，Valine抗性基因(ilvG；编码乙酰羟基酸合成酶)中存在一个移码位点。IlvG+2096(以前命名为ilv02096)突变取代了这个移码位点，导致ilvG的表达，并缓解了该操纵子远端基因的转录极性。因此，ilv0突变，伴随着valine抗性和ilvEDA基因表达的增加，是ilvGEDA操纵子第一结构基因内的一个极性位置的逆转。
The relationship of valine resistance to the expression of the ilvGEDA operon of E. coli K-12 was determined. DNA sequence and in vivo protein analyses indicate that, in wild-type E. coli K-12, there is a frameshift site within the gene (ilvG; which codes for acetohydroxy acid synthase) for valine resistance. The ilvG+2096 (formerly designated ilv02096) mutation displaces this frameshift site, resulting in the expression of ilvG and the relief to transcriptional polarity on the distal genes of this operon. Thus, the ilv0 mutation, which concomitantly confers valine resistance and increased expression of the ilvEDA genes, is the reversion of a polar site within the 1st structural gene of the ilvGEDA operon.