Glucosamine-modified polyethylene glycol hydrogel-mediated chondrogenic differentiation of human mesenchymal stem cells
Glucosamine-modified polyethylene glycol hydrogel-mediated chondrogenic differentiation of human mesenchymal stem cells
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氨基葡萄糖修饰的聚乙二醇水凝胶介导人间充质干细胞的软骨分化
DOI：
10.1016/j.msec.2017.05.043
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发表时间：
2017-10-01
影响因子：
7.9
通讯作者：
Ren, Li
中科院分区：
文献类型：
--
作者：
Yao, Hang;Xue, Jingchen;Ren, Li
氨基葡萄糖（Glucosamine，GA）是一种重要的软骨基质前体，可促进软骨再生，尤其是在骨关节炎的治疗中。然而，它还没有被广泛用作软骨修复支架的生物活性基团。本研究采用氨基葡萄糖修饰聚乙二醇（PEG）水凝胶，将人骨髓间充质干细胞（hBMSCs）包裹于PEG水凝胶中，在三维培养条件下诱导hBMSCs向软骨细胞分化。GA修饰的PEG水凝胶促进hBMSCs的软骨形成，特别是在5 mM和10 mM的浓度下。10 mM GA修饰的水凝胶与hBMSCs皮下移植8周形成软骨样块。重要的是，随着葡糖胺的增加，改性水凝胶在蛋白质水平下调纤维化和肥大软骨标志物。因此，葡萄糖胺修饰的PEG水凝胶促进hBMSCs的软骨形成，这可能代表了一种新的方法，软骨修复使用组织工程的方法。(C)2017由Elsevier B.V.出版
Glucosamine (GA) is an important cartilage matrix precursor for the glycosaminoglycan biochemical synthesis, and has positive effects on cartilage regeneration, particularly in osteoarthritis therapy. However, it has not been used as a bioactive group in scaffolds for cartilage repair widely. In this study, we synthesized modified polyethylene glycol (PEG) hydrogel with glucosamine and then encapsulated human bone mesenchymal stem cells (hBMSCs) in the hydrogel to induce the differentiation of hBMSCs into chondrocytes in three-dimensional culture. The GA-modified PEG hydrogels promoted the chondrogenesis of hBMSCs, particularly in the concentration of 5 mM and 10 mM. The subcutaneous transplantation of 10 mM GA-modified hydrogels with hBMSCs formed cartilage-like blocks in vivo for 8 weeks. Importantly, with glucosamine increase, the modified hydrogels down regulated the fibrosis and hypertrophic cartilage markers in protein level. Therefore, glucosamine modified PEG hydrogels facilitated the chondrogenesis of hBMSCs, which might represent a new method for cartilage repair using a tissue-engineering approach. (C) 2017 Published by Elsevier B.V.