Molecular phylogenetic, morphological, and mycotoxin data support reidentification of the Quorn mycoprotein fungus as Fusarium venenatum
Molecular phylogenetic, morphological, and mycotoxin data support reidentification of the Quorn mycoprotein fungus as Fusarium venenatum
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DOI：
10.1006/fgbi.1997.1018
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发表时间：
1998-02-01
影响因子：
3
通讯作者：
Casper, HH
中科院分区：
文献类型：
--
作者：
O'Donnell, K;Cigelnik, E;Casper, HH
为了研究镰刀菌中Quorn真菌蛋白真菌的关系和鉴定，并检验Quorn菌株和商业Quorn食品中的霉菌毒素，获得了分子系统发育、形态和真菌毒素数据。通过聚合酶链反应从核28S核糖体DNA、核糖体内部转录间隔区获得的DNA序列进行了系统发育分析。β -微管蛋白基因外显子和内含子表明，Quorn真菌是镰刀菌（Fusarium venenatum），而不是以前报道的F. graminearum。除NRRL 25139外，所有的Quorn菌株在形态上都是退化的孢子菌落突变体，NRRL 25139产生的衣原体孢子在弯曲的末端链上，并在诊断为venenatum的单孢单孢体上产生大多数5分离的孢子孢子。Bootstrap和腐烂分析强有力地支持了含有F. venenatum和其他几种产生a型毛霉烯的物种的单系谱系，而F. guaminearum的参考菌株则嵌套在产生B型毛霉烯和/或玉米赤霉烯酮的一个单独的物种分支中。但与毒株NRRL 22198相比含量较低。然而，在英国销售供人类消费的三种商业Quorn产品的分析中未检测到毛霉烯真菌毒素。(C) 1998学术出版社。
Molecular phylogenetic, morphological, and mycotoxin data were obtained in order to investigate the relationships and identity of the Quorn mycoprotein fungus within Fusarium and to examine Quorn strains and commercial Quorn food products for trichothecene mycotoxins, Phylogenetic analyses of aligned DNA sequences obtained via the polymerase chain reaction from the nuclear 28S ribosomal DNA, nuclear ribosomal internal transcribed spacer region, and beta-tubulin gene exons and introns indicate that the Quorn fungus is Fusarium venenatum, rather than F. graminearum as previously reported. All of the Quorn strains examined were morphologically degenerate aconidial colonial mutants except for NRRL 25139, which produced chlamydospores in recurved terminal chains together with mostly 5-septate sporodochial conidia on doliform monophialides diagnostic of F. venenatum. Bootstrap and decay analyses provide strong support for a monophyletic lineage containing F. venenatum and several other type A trichothecene-producing species, while reference strains of F. guaminearum were nested in a separate clade of species that produce type B trichothecenes and/or zearalenone, Analysis of mycotoxins from rice cultures inoculated with Quorn strain NRRL 25416 revealed that four type A trichothecenes are produced, but at low levels relative to strain NRRL 22198 of F. venenatum. No trichothecene mycotoxins, however, were detected from the analysis of three commercial Quorn products marketed for human consumption in England. (C) 1998 Academic Press.