RETINOIC ACID INDUCES NEURONAL DIFFERENTIATION OF A CLONED HUMAN EMBRYONAL CARCINOMA CELL-LINE INVITRO
RETINOIC ACID INDUCES NEURONAL DIFFERENTIATION OF A CLONED HUMAN EMBRYONAL CARCINOMA CELL-LINE INVITRO
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DOI：
10.1016/0012-1606(84)90316-6
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发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
ANDREWS, PW
中科院分区：
文献类型：
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作者：
ANDREWS, PW
从Tera-2克隆衍生的人胚胎癌细胞NT2/D1和NT2/B9在体外暴露于维甲酸时广泛分化。这种分化的特征是几种形态不同的细胞类型的出现和细胞表面表型的变化，特别是人类EC细胞特有表达的阶段特异性胚胎抗原-3 （SSEA-3）的消失。在分化的细胞中有神经元，它们通过轴突束的扩展网络相互连接形成簇，并表达破伤风毒素受体和神经丝蛋白。这些观察结果构成了体外克隆人类EC细胞系广泛体细胞分化的第一个实例。
The human embryonal carcinoma cell lines NT2/D1 and NT2/B9, clonally derived from Tera-2, differentiate extensively in vitro when exposed to retinoic acid. This differentiation is marked by the appearance of several morphologically distinct cell types and by changes in cell surface phenotype, particularly by the disappearance of stage-specific embryonic antigen-3 (SSEA-3), which is characteristically expressed by human EC cells. Among the differentiated cells are neurons, which form clusters interconnected by extended networks of axon bundles, and which express tetanus toxin receptors and neurofilament proteins. These observations constitute the 1st instance of extensive somatic differentiation of a clonal human EC cell line in vitro.