SGI-4 in monophasic Salmonella Typhimurium ST34 is a novel ICE that enhances resistance to copper
SGI-4 in monophasic Salmonella Typhimurium ST34 is a novel ICE that enhances resistance to copper
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单相鼠伤寒沙门氏菌 ST34 中的 SGI-4 是一种新型 ICE，可增强对铜的抵抗力
DOI：
10.1101/518175
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Branchu P
中科院分区：
文献类型：
--
作者：
Branchu P
序列类型 34 的多重耐药性肠沙门氏菌4,[5],12:i-（单相鼠伤寒沙门氏菌 ST34）是目前与牲畜（特别是猪）以及人群中多次爆发有关的大流行克隆。一个大的基因组岛，称为 SGI-4，存在于单相鼠伤寒 ST34 进化枝中，而在其他进化枝中不存在。鼠伤寒菌株。 SGI-4 由 87 个开放阅读框组成，包括先前涉及铜 (Cu) 和银抗性的 silandpcgenes，以及预计通过接合参与动员和转移的多个基因。将SGI-4从染色体上切除、环化并转移至S.的受体菌株中。鼠伤寒菌的频率受丝裂霉素 C 诱导的应激和氧张力的影响。 SGI-4 的存在与铜抗性增加有关，特别是但不仅限于在厌氧条件下。 silCBA基因的存在，预计编码RND家族外排泵，将铜从周质转运到外部环境，足以赋予观察到的对铜的增强的抗性，高于通常与S相关的抗性。鼠伤寒分离株。这些基因的存在导致缺乏铜依赖性诱导，编码与铜抗性相关的多种蛋白质的pc基因也存在于SGI-4上，表明该系统有效地限制了周质中铜的可用性，但不影响SodCI依赖性巨噬细胞的存活。
A multi drug resistantSalmonella enterica4,[5],12:i- of sequence type 34 (monophasicS. Typhimurium ST34) is a current pandemic clone associated with livestock, particularly pigs, and numerous outbreaks in the human population. A large genomic island, termed SGI-4, is present in the monophasic Typhimurium ST34 clade and absent from otherS. Typhimurium strains. SGI-4 consists of 87 open reading frames includingsilandpcogenes previously implicated in resistance to copper (Cu) and silver, and multiple genes predicted to be involved in mobilization and transfer by conjugation. SGI-4 was excised from the chromosome, circularized, and transferred to recipient strains ofS. Typhimurium at a frequency influenced by stress induced by mitomycin C, and oxygen tension. The presence of SGI-4 was associated with increased resistance to Cu, particularly but not exclusively under anaerobic conditions. The presence ofsilCBAgenes, predicted to encode an RND family efflux pump that transports Cu from the periplasm to the external milieu, was sufficient to impart the observed enhanced resistance to Cu, above that commonly associated withS. Typhimurium isolates. The presence of these genes resulted in the absence of Cu-dependent induction ofpcogenes encoding multiple proteins linked to Cu resistance, also present on SGI-4, suggesting that the system effectively limits the Cu availability in the periplasm, but did not affect SodCI-dependent macrophage survival.