Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana.
Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana.
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DOI：
10.1186/1743-422x-9-78
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发表时间：
2012-04-10
期刊：
影响因子：
4.8
通讯作者：
Owusu M
中科院分区：
文献类型：
--
作者：
Kwofie TB;Anane YA;Nkrumah B;Annan A;Nguah SB;Owusu M
急性呼吸道感染是发展中国家幼儿发病和死亡的主要原因之一。关于发展中国家急性呼吸道感染病毒病因的信息非常有限。这项研究是为了确定与5岁以下儿童急性下呼吸道感染相关的病毒。鼻咽样本和血培养收集自因急性下呼吸道感染住院的5岁以下儿童。病毒和细菌进行了鉴定，使用逆转录酶实时聚合酶链反应和传统的生物化学技术。在招募的128例患者中，33例（25.88%，95%CI：18.5%至34.2%）对一种或多种病毒呈阳性。18例患者检出呼吸道合胞病毒（RSV）（14.1%，95%CI：8.5%至21.3%），13例患者随后接受腺病毒（AdV）治疗（10.2%，95%CI：5.5%至16.7%），副流感病毒（PIV类型：1、2、3）4例（3.1%，95%CI：0.9%至7.8%），流感B病毒1例（0.8%，95%CI：0.0至4.3）。2例患者同时发生病毒和细菌合并感染。对于分离的各种病原体，在临床体征、症状和严重程度方面没有可检测到的显著差异。雨季病毒检出率为61.1%（22/36），以呼吸道合胞病毒为主。该研究表明，呼吸道病毒是加纳三级卫生机构儿童急性呼吸道感染的主要原因。这些数据解决了对病毒相关呼吸道感染进行更多研究的需求。
Acute respiratory tract infections are one of the major causes of morbidity and mortality among young children in developing countries. Information on the viral aetiology of acute respiratory infections in developing countries is very limited. The study was done to identify viruses associated with acute lower respiratory tract infection among children less than 5 years. Nasopharyngeal samples and blood cultures were collected from children less than 5 years who have been hospitalized for acute lower respiratory tract infection. Viruses and bacteria were identified using Reverse Transcriptase Real-Time Polymerase Chain Reaction and conventional biochemical techniques. Out of 128 patients recruited, 33(25.88%%, 95%CI: 18.5% to 34.2%) were positive for one or more viruses. Respiratory Syncytial Virus (RSV) was detected in 18(14.1%, 95%CI: 8.5% to 21.3%) patients followed by Adenoviruses (AdV) in 13(10.2%, 95%CI: 5.5% to 16.7%), Parainfluenza (PIV type: 1, 2, 3) in 4(3.1%, 95%CI: 0.9% to 7.8%) and influenza B viruses in 1(0.8%, 95%CI: 0.0 to 4.3). Concomitant viral and bacterial co-infection occurred in two patients. There were no detectable significant differences in the clinical signs, symptoms and severity for the various pathogens isolated. A total of 61.1% (22/36) of positive viruses were detected during the rainy season and Respiratory Syncytial Virus was the most predominant. The study has demonstrated an important burden of respiratory viruses as major causes of childhood acute respiratory infection in a tertiary health institution in Ghana. The data addresses a need for more studies on viral associated respiratory tract infection.