Elastic volume reconstruction from series of ultra-thin microscopy sections
Elastic volume reconstruction from series of ultra-thin microscopy sections
复制标题
DOI：
10.1038/nmeth.2072
复制
发表时间：
2012-07-01
期刊：
影响因子：
48
通讯作者：
Tomancak, Pavel
中科院分区：
文献类型：
--
作者：
Saalfeld, Stephan;Fetter, Richard;Tomancak, Pavel
大型生物标本的解剖结构通常由高分辨率显微镜成像的连续切片体积重建。我们开发了一种方法来重新组装一个连续的体积，从这样的大截面系列，明确地最大限度地减少人为变形，通过应用一个全球性的弹性约束。我们证明了我们的方法上的一系列透射电子显微镜切片覆盖整个558细胞秀丽隐杆线虫胚胎和果蝇幼虫腹神经索的一部分。
Anatomy of large biological specimens is often reconstructed from serially sectioned volumes imaged by high-resolution microscopy. We developed a method to reassemble a continuous volume from such large section series that explicitly minimizes artificial deformation by applying a global elastic constraint. We demonstrate our method on a series of transmission electron microscopy sections covering the entire 558-cell Caenorhabditis elegans embryo and a segment of the Drosophila melanogaster larval ventral nerve cord.