INSULIN INHIBITS SPECIFIC NOREPINEPHRINE UPTAKE IN NEURONAL CULTURES FROM RAT-BRAIN
INSULIN INHIBITS SPECIFIC NOREPINEPHRINE UPTAKE IN NEURONAL CULTURES FROM RAT-BRAIN
复制标题
DOI：
10.1016/0006-8993(86)91242-4
复制
发表时间：
1986-11-19
期刊：
影响因子：
2.9
通讯作者：
RAIZADA, MK
中科院分区：
文献类型：
--
作者：
BOYD, FT;CLARKE, DW;RAIZADA, MK
已经证明原代培养的神经元细胞具有特异性胰岛素受体（Boyd等人，J. Biol. Chem.，260（1985）15880-15884）。这些培养物与胰岛素孵育导致马普替林敏感性[3 H]去甲肾上腺素摄取的剂量依赖性抑制。在胰岛素浓度为167 nM时观察到95%的马普替林敏感性去甲肾上腺素摄取的最大抑制，ED 50为30 nM。竞争抑制和Scatchard分析的胰岛素结合数据表明，马普替林竞争高亲和力的胰岛素受体。这些观察结果表明，胰岛素和马普替林特异性抑制神经元去甲肾上腺素摄取的可能性，涉及胰岛素受体。
Neuronal cells in primary culture have been demonstrated to possess specific insulin receptors (Boyd et al., J. Biol. Chem., 260 (1985) 15880-15884). Incubation of these cultures with insulin causes a dose-dependent inhibition of maprotiline-sensitive [3H]norepinephrine uptake. Maximum inhibition of 95% of maprotiline-sensitive norepinephrine uptake ws observed at an insulin concentration of 167 nM with an ED50 of 30 nM. Competition-inhibition and Scatchard analysis of the insulin binding data suggested that maprotiline competed for high-affinity insulin receptors. These observations suggest that both insulin and maprotiline specifically inhibit neuronal norepinephrine uptake possibility involving insulin receptors.