Analysis of genetic factors influencing the developmental rate of globally important CIMMYT wheat cultivars
Analysis of genetic factors influencing the developmental rate of globally important CIMMYT wheat cultivars
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DOI：
10.2135/cropsci2004.0665
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发表时间：
2005-09-01
期刊：
影响因子：
2.3
通讯作者：
Worland, AJ
中科院分区：
文献类型：
--
作者：
van Beem, J;Mohler, V;Worland, AJ
小麦（Triticum aestivum L.）开花期和其他发育阶段的早熟性主要取决于春化作用（Vrn）、光周期（Ppd）和早熟性本身（Eps）这三个主要因子的变化。本研究的目的是表征一组基因型的存在下，发育基因和它们的潜在关联的适应性，并确定诊断分子标记的春化基因Vrn-D1。51个基因型与含有已知Vrn和Ppd基因的测试者杂交。为了确定早熟本身及其与温度的相互作用，基因型春化8周，并放置在一个24小时的光周期制度在两个温度。为了验证Vrn-D1的分子标记，用紧密连锁的SSR标记Xgwm 292 -5D和Xgwm 212 -5D对这些品种进行基因分型。田间生长的植物的F-2群体的分离分析表明，Vrn-D1存在于66%的品种，而Vrn-A1，Vrn-B1和Vrn 4被发现在41，39和8%的线，分别单独或与其他Vrn基因组合。一个低比例的品种似乎有一个组成型的遗传性状早开花（Eps），而大多数的品种可能有温度敏感的基因，响应当地的环境变化。利用微卫星标记Xgwm 292 -5D的215-bp等位基因，在76.5%的基因型中可靠地检测到Vrn-D1，而Xgwm 212 -5D没有显示出足够的等位基因变异。
The rate at which wheat (Triticum aestivum L.) reaches anthesis and other developmental stages depends largely on the variation of three major factors: vernalization (Vrn), photoperiod (Ppd), and earliness per se (Eps). The objectives of this study were to characterize a set of genotypes for the presence of developmental genes and their potential association to adaptability and to identify diagnostic molecular markers for the vernalization gene Vrn-D1. Fifty-one genotypes were crossed with testers containing known Vrn and Ppd genes. To determine earliness per se and its interaction with temperature, genotypes were vernalized for 8 wk and placed in a 24-h photoperiod regime at two temperatures. To validate molecular markers for Vrn-D1, the cultivars were genotyped with closely linked SSR markers Xgwm292-5D and Xgwm212-5D. Segregation analysis on F-2 populations of field-grown plants showed that Vrn-D1 was present in 66% of the cultivars, while Vrn-A1, Vrn-B1, and Vrn4 were found in 41, 39, and 8% of the lines, respectively, either solely or in combination with other Vrn genes. A low percentage of cultivars appeared to have a constitutive genetic character for early flowering (Eps), while most of the cultivars may have temperature-sensitive genes that respond to local environmental changes. Vrn-D1 was reliably detected in 76.5% of the genotypes using the 215-bp allele of microsatellite marker Xgwm292-5D, whereas Xgwm212-5D did not reveal sufficient allelic variation within the tested wheats.