Regulation Effects of Crataegus pinnatifida Leaf on Glucose and Lipids Metabolism
Regulation Effects of Crataegus pinnatifida Leaf on Glucose and Lipids Metabolism
复制标题
DOI：
10.1021/jf1049062
复制
发表时间：
2011-05-11
影响因子：
6.1
通讯作者：
Zhang, Yi
中科院分区：
文献类型：
--
作者：
Wang, Tao;An, Yating;Zhang, Yi
山楂叶为蔷薇科植物，中国常生食或熟食，以促进消化、促进血液循环。为了研究其对糖脂代谢的调节作用，制备了黄酮类成分，并用高效液相色谱和液质联用对其进行了分析。在体内，250和500 mg/kg剂量的黄酮组分对甘油三酯和葡萄糖吸收有抑制作用，对胃肠转运有促进作用，但对胃排空无影响。体外培养的3T3-L1前脂肪细胞经30微克/毫升的黄酮组分处理后，甘油三酯和游离脂肪酸的积聚明显受到抑制。抑制C/EBPα、PPAR-γ、SREBP1c、aP2和脂联素的基因表达，但不影响瘦素的基因表达。羽扇叶可能对2型糖尿病和高脂血症患者有用，作为一种食物。
The leaf of Crataegus pinnatifida (Rosaceae) is commonly consumed either raw or cooked to improve digestion and promote blood circulation in China. To investigate the regulation effects of it on glucose and lipid metabolism, the flavonoids fraction was prepared and analyzed by HPLC and LC-MS. In vivo, at doses of 250 and 500 mg/kg, the flavonoids fraction showed inhibitory effects on TG and glucose absorption, accelerating effects on gastrointestinal transit but no effect on gastric emptying. In vitro, treatment of 3T3-L1 preadipocytes with 30 mu g/mL flavonoids fraction significantly suppressed the accumulation of TG and free fatty acid. It also suppressed the gene expressions of C/EBP alpha, PPAR gamma, SREBP 1c, aP2 and adiponectin but did not affect that of leptin. C. pinnatifida leaf may be useful for type 2 diabetics and hyperlipidemics as a foodstuff.