The blood-brain barrier is continuously open for several weeks following transient focal cerebral ischemia
The blood-brain barrier is continuously open for several weeks following transient focal cerebral ischemia
复制标题
DOI：
10.1016/j.neuroscience.2008.02.012
复制
发表时间：
2008-04-22
期刊：
影响因子：
3.3
通讯作者：
Tatlisumak, T.
中科院分区：
文献类型：
--
作者：
Strbian, D.;Durukan, A.;Tatlisumak, T.
血脑屏障(BIBB)是血源性物质进入脑实质的主要调节因子。因此，在脑缺血、炎症、创伤和肿瘤等病理条件下，血脑屏障渗漏是常见的，导致脑水肿和有毒物质的涌入。长期以来，BIBB在缺血再灌注损伤后的渗漏一直被认为是双相的，尽管在第二次开放的时间上在研究中确实存在相当大的分歧。这使得我们使用Gd增强(小分子)磁共振成像和荧光染料Evans Blue(大分子)，系统地和定量地评估了90分钟缺血再灌注大鼠模型中Bibb渗漏的动力学。分别于再灌注后25min、2、4、6、12、18、24、36、48、72 h，1、2、3、4、5周观察BIBB渗漏情况。我们早在再灌流后25分钟就观察到Gd和Evans Blue的Bibb渗漏。此后，BBB对Evans Blue的开放时间长达3周，对Gd的开放时间长达5周。结果表明，大鼠脑缺血再灌注损伤后Bibb渗漏是持续的，持续时间长，最长可达数周。这是第一个全面展示短暂性脑缺血后Bibb渗漏动力学的系统和广泛的研究，其发现具有重要的临床和实验意义。(C)2008年IBRO。爱思唯尔有限公司出版。保留所有权利。
The blood-brain barrier (BIBB) is the principal regulator of blood-borne substance entry into the brain parenchyma. Therefore, BBB leakage, which leads to cerebral edema and influx of toxic substances, is common in pathological conditions such as cerebral ischemia, inflammation, trauma, and tumors. The leakage of BIBB after ischemia-reperfusion injury has long been considered to be biphasic, although a considerable amount of discrepancies as for the timing of the second opening does exist among the studies. This led us to evaluate systematically and quantitatively the dynamics of BIBB leakage in a rat model of 90-min ischemia-reperfusion, using gadolinium-enhanced (small molecule) magnetic resonance imaging and fluorescent dye Evans Blue (large molecule). BIBB leakage was assessed at the following time points after reperfusion: 25 min, 2, 4, 6, 12, 18, 24, 36, 48, and 72 h, and 1, 2, 3, 4, and 5 weeks. We observed BIBB leakage for both gadolinium and Evans Blue as early as 25 min after reperfusion. Thereafter, BBB remained open for up to 3 weeks for Evans Blue and up to 5 weeks for gadolinium. Our results show that BIBB leakage after ischemia-reperfusion injury in the rat is continuous and long-lasting, without any closure up to several weeks. This is the first systematic and extensive study fully demonstrating BIBB leakage dynamics following transient brain ischemia and the findings are of major clinical and experimental interest. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.