Fluid mechanics of a spinner-flask bioreactor
Fluid mechanics of a spinner-flask bioreactor
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DOI：
10.1002/bit.10788
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发表时间：
2004-01-05
影响因子：
3.8
通讯作者：
Neitzel, GP
中科院分区：
文献类型：
--
作者：
Sucosky, P;Osorio, DF;Neitzel, GP
转瓶生物反应器已被用于生产关节软骨在体外。已知生物反应器内的动态环境显著影响组织的生长和发育。本研究的重点是在用于生产软骨的条件下操作的旋转烧瓶内流场的实验和数值表征。在一个按比例放大的模型生物反应器中进行的实验室实验采用粒子图像测速（PIV），以确定速度和剪切速率场附近的结构最接近的搅拌棒，除了湍流特性。利用商业软件包FLUENT进行数值计算，模拟了相同模型生物反应器在相似操作条件下的流场。在计算中，支架被建模为具有不同渗透率的固体和多孔介质，并检查了通过最靠近搅拌棒的构造的各个面的流速。(C)2004 Wiley Periodicals，Inc.
Spinner-flask bioreactors have been used for the production of articular cartilage in vitro. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. The present research focuses on the experimental and numerical characterization of the flow field within a spinner flask operating under conditions used to produce cartilage. Laboratory experiments carried out in a scaled-up model bioreactor employ particle-image velocimetry (PIV) to determine velocity and shear-rate fields in the vicinity of the construct closest to the stir bar, in addition to turbulence properties. Numerical computations calculated using FLUENT, a commercial software package, simulate the flow field in the same model bioreactor under similar operating conditions. In the computations, scaffolds were modeled as both solid and porous media with different permeabilities and flow rates through various faces of the construct nearest the stir bar were examined. (C) 2004 Wiley Periodicals, Inc.