SARS-CoV-2 neutralization and serology testing of COVID-19 convalescent plasma from donors with nonsevere disease.
SARS-CoV-2 neutralization and serology testing of COVID-19 convalescent plasma from donors with nonsevere disease.
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DOI：
10.1111/trf.16101
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发表时间：
2021-01
期刊：
影响因子：
2.9
通讯作者：
Bold TD
中科院分区：
文献类型：
--
作者：
Gniadek TJ;Thiede JM;Matchett WE;Gress AR;Pape KA;Fiege JK;Jenkins MK;Menachery VD;Langlois RA;Bold TD
用恢复期血浆转移被动免疫是治疗和预防COVID-19的一种有前景的策略，但有非严重疾病史的供体在血清学上是异质的。SARS-Cov-2抗原结合活性和中和活性之间的关系尚未确定。使用三种临床诊断血清学检测方法（Beckman、DiaSorin和Roche）对47名有非严重COVID-19病史的个体的恢复期血浆单位进行了抗原结合活性评估，这些检测方法具有不同的SARS-CoV-2靶标。将这些结果与在微孔测定中使用SARS-CoV-2的荧光报告菌株的功能性中和活性进行比较。确定了抗原结合和病毒中和之间不同强度（斯皮尔曼r = 0.37-0.52）的正相关性。年龄在48 - 75岁之间的供体具有最高的中和活性。富集（75%-82%）各试验中结合活性最高三分位数的单位，以获得最高中和水平。抗原结合活性和中和之间的关系强度取决于所用的临床测定。各试验中结合活性最高三分位数的单位主要由具有最大中和活性的单位组成。
The transfer of passive immunity with convalescent plasma is a promising strategy for treatment and prevention of COVID-19, but donors with a history of nonsevere disease are serologically heterogenous. The relationship between SARS-Cov-2 antigen-binding activity and neutralization activity in this population of donors has not been defined. Convalescent plasma units from 47 individuals with a history of nonsevere COVID-19 were assessed for antigen-binding activity of using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS-CoV-2 targets. These results were compared with functional neutralization activity using a fluorescent reporter strain of SARS-CoV-2 in a microwell assay. Positive correlations of varying strength (Spearman r = 0.37–0.52) between antigen binding and viral neutralization were identified. Donors age 48 to 75 years had the highest neutralization activity. Units in the highest tertile of binding activity for each assay were enriched (75%–82%) for those with the highest levels of neutralization. The strength of the relationship between antigen-binding activity and neutralization varies depending on the clinical assay used. Units in the highest tertile of binding activity for each assay are predominantly comprised of those with the greatest neutralization activity.