Linear and nonlinear mouse sperm motility patterns. A quantitative classification.
Linear and nonlinear mouse sperm motility patterns. A quantitative classification.
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线性和非线性小鼠精子运动模式。
DOI：
10.1002/j.1939-4640.1985.tb00813.x
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发表时间：
1985
影响因子：
--
通讯作者：
Olds-Clarke,P
中科院分区：
文献类型：
--
作者：
Tessler,S;Olds-Clarke,P
本文提出了一种新的参数--线性指数，用于区分两种小鼠精子活力的定量方法。使用来自C57 BL/6-+/+小鼠的100个精子和来自C57 BL/6-tw 32/+小鼠的100个精子评价该参数。通过视频显微摄影术跟踪精子，并计算以下参数：净位移速度Vn;精子头部曲线速度Vc;“平均”速度Va（轨迹的5点移动平均值）;进行性比率（Vn/Vc）;曲线进行性比率（Va/Vc）;和线性指数（Vn/Va）。两组线性指数值的频率直方图均为双峰，并且在0.5处各有一个明确的反模，将精子的两个离散亚群分开。在每组内，“线性”（>0.5）和“非线性”（<0.5）轨迹在所有特征上都不同;组间，线性精子相似，非线性精子仅在精子头部的曲线速度上不同。
A quantitative method is described for distinguishing two types of mouse sperm motility using a new parameter, the Linear Index. One hundred spermatozoa from C57BL/6‐+/+ and 100 spermatozoa from C57BL/6‐tw32/+ mice were used to evaluate this parameter. Spermatozoa were tracked by videomicrography, and the following calculated: net displacement velocity, Vn; curvilinear velocity of the sperm head, Vc; the “average” velocity, Va (a 5‐point moving average of the track); the progressiveness ratio, (Vn/Vc); the curvilinear progressiveness ratio, (Va/Vc); and a linear index, (Vn/Va). Frequency histograms of linear index values were bimodal for both groups and each had a clear antimode at 0.5, separating two discrete subpopulations of spermatozoa. Within each group, “linear” (>0.5) and “nonlinear” (<0.5) tracks differed in all characteristics; between groups, linear spermatozoa were similar and nonlinear spermatozoa differed only in the curvilinear velocity of the sperm head.