Renal responses to intra-arterial administration of nitric oxide donor in dogs.
Renal responses to intra-arterial administration of nitric oxide donor in dogs.
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DOI：
10.1161/01.hyp.22.4.535
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发表时间：
1993-10
期刊：
影响因子：
8.3
通讯作者：
D. S. Majid;A. Williams;P. Kadowitz;L. Navar
中科院分区：
文献类型：
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作者：
D. S. Majid;A. Williams;P. Kadowitz;L. Navar
通过动脉内给予硝基-L-精氨酸（NLA）抑制一氧化氮合成导致肾动脉压（RAP）和钠排泄之间关系的斜率衰减，而不改变肾自动调节效率。在本研究中，我们研究是否只有一氧化氮的存在，或者，在RAP的变化过程中，一氧化氮的产生变化是需要压力尿钠排泄发生。用NLA（50 μ g.kg-1 x min-1）处理麻醉的钠充足犬（n = 8）以抑制内源性一氧化氮形成，并以恒定速率（2 μ g.kg-1 x min-1）动脉内输注S-亚硝基-n-乙酰青霉胺（SNAP）以补充肾内一氧化氮水平。在NLA输注前和输注期间，随后进行SNAP+NLA输注，评估了在自身调节范围内RAP降低的肾脏反应。如前所述，NLA单独输注增加了肾血管阻力，降低了肾血流量、尿流量、钠排泄和钠排泄分数，但肾小球滤过率无变化。自动调节效率保持不变，而压力诱导的利钠反应减弱。在SNAP+NLA输注期间，肾血流量从2.8 +/- 0.3增加至3.5 +/- 0.3 mL.min-1 x g-1（P < .001），肾小球滤过率无显著变化（0.75 +/- 0.07至0.81 +/- 0.05 mL.min-1 x g-1）;肾血流量和肾小球滤过率的自动调节效率保持不变。SNAP增加尿流量（4.8 +/- 1.8至10.0 +/- 2.5 microL.min-1 x g-1）、钠排泄（0.63 +/- 0.26至1.70 +/- 0.37 mumol.min-1 x g-1）和钠排泄分数（0.55 +/- 0.20%至1.38 +/- 0.27%）。（250字处删节）
Inhibition of nitric oxide synthesis by intra-arterial administration of nitro-L-arginine (NLA) leads to attenuation of the slope of the relation between renal arterial pressure (RAP) and sodium excretion without an alteration in renal autoregulatory efficiency. In the present study, we examined whether only the presence of nitric oxide or, alternatively, changes in nitric oxide production during changes in RAP are required for pressure natriuresis to occur. Anesthetized sodium-replete dogs (n = 8) were treated with NLA (50 micrograms.kg-1 x min-1) to inhibit endogenous nitric oxide formation, and S-nitroso-n-acetylpenicillamine (SNAP) was infused intra-arterially at a constant rate (2 micrograms.kg-1 x min-1) to replenish intrarenal nitric oxide levels. Renal responses to reductions in RAP within the autoregulatory range were assessed before and during NLA infusion followed by SNAP+NLA infusion. As reported previously, NLA infusion alone increased renal vascular resistance and decreased renal blood flow, urine flow, sodium excretion, and fractional excretion of sodium, with no change in glomerular filtration rate. Autoregulatory efficiency remained intact, whereas the pressure-induced natriuretic responses were attenuated. During SNAP+NLA infusion, renal blood flow increased from 2.8 +/- 0.3 to 3.5 +/- 0.3 mL.min-1 x g-1 (P < .001), without significant changes in glomerular filtration rate (0.75 +/- 0.07 to 0.81 +/- 0.05 mL.min-1 x g-1); the autoregulatory efficiency of renal blood flow and glomerular filtration rate remained intact. SNAP increased urine flow (4.8 +/- 1.8 to 10.0 +/- 2.5 microL.min-1 x g-1), sodium excretion (0.63 +/- 0.26 to 1.70 +/- 0.37 mumol.min-1 x g-1), and fractional excretion of sodium (0.55 +/- 0.20% to 1.38 +/- 0.27%).(ABSTRACT TRUNCATED AT 250 WORDS)