Molecular characterization of the liver-expressed antimicrobial peptide 2 (LEAP-2) in a teleost fish, Plecoglossus altivelis: Antimicrobial activity and molecular mechanism
Molecular characterization of the liver-expressed antimicrobial peptide 2 (LEAP-2) in a teleost fish, Plecoglossus altivelis: Antimicrobial activity and molecular mechanism
复制标题
硬骨鱼 Plecoglossus altivelis 肝脏表达的抗菌肽 2 (LEAP-2) 的分子特征：抗菌活性和分子机制
DOI：
10.1016/j.molimm.2015.02.022
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发表时间：
2015-06-01
影响因子：
3.6
通讯作者：
Chen, Jiong
中科院分区：
文献类型：
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作者：
Li, He-Xiang;Lu, Xin-Jiang;Chen, Jiong
肝脏表达的抗菌肽2（LEAP-2）广泛存在于鱼类体内，在宿主的天然免疫系统中起着重要作用。本研究克隆了香鱼LEAP-2（PaLEAP-2）基因的全长cDNA并进行了序列测定。在广泛的组织中检测到PaLEAP-2 mRNA，在肝脏中发现的转录物水平最高。经鳗弧菌诱导后，其在肝脏、肾脏、脾脏、鳃和心脏中的表达显著增加，但在肠道中的表达降低。化学合成的PaLEAP-2成熟肽，它表现出对各种细菌的体外选择性抗菌活性。高浓度PaLEAP-2可降低鳗弧菌感染香鱼的细菌负荷，提高其存活率。此外，在高浓度和低浓度下，它抑制了鳗弧菌感染的香鱼中TNF-α和IL-1 β mRNA的表达。PaLEAP-2诱导pET-22 b质粒DNA和细菌基因组DNA水解。这些结果表明PaLEAP-2在香鱼抗细菌感染的免疫应答中起作用。(C)2015爱思唯尔有限公司版权所有。
Liver-expressed antimicrobial peptide 2 (LEAP-2) is widespread in fish and plays an important role in the host's innate immune system. In this study, full-length cDNA for LEAP-2 (PaLEAP-2) gene was cloned and sequenced from ayu, Plecogiossus altivelis. PaLEAP-2 mRNA was detected in a wide range of tissues, with the highest level of transcripts found in the liver. Upon induction by Vibrio anguillarum, its expression significantly increased in the liver, kidney, spleen, gill, and heart, but decreased in the intestine. The PaLEAP-2 mature peptide was chemically synthesized; it exhibited selective antimicrobial activity against various bacteria in vitro. PaLEAP-2 at high concentration reduced the bacterial load and improved the survival rate of V. anguillarum-infected ayu. Moreover, it inhibited the expression of mRNAs for TNF-alpha and IL-1 beta in V. anguillarum-infected ayu, both at high and low concentrations. PaLEAP-2 induced hydrolysis of the pET-22b plasmid DNA and bacterium genomic DNA. These results suggest that PaLEAP-2 plays a role in ayu immune responses against bacterial infection. (C) 2015 Elsevier Ltd. All rights reserved.