Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span
Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span
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DOI：
10.1016/j.mad.2010.04.003
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发表时间：
2010-05-01
影响因子：
5.3
通讯作者：
Buerkle, Alexander
中科院分区：
文献类型：
--
作者：
Beneke, Sascha;Scherr, Anna-Lena;Buerkle, Alexander
聚（ADP-核糖基）化是一种翻译后修饰，其参与许多细胞功能，包括DNA修复和基因组稳定性的维持，并且还与细胞和生物体衰老有关。我们以前曾报道，最大聚（ADP-核糖基）化能力在单核血细胞与哺乳动物的寿命。在这里，我们表明，长寿和短命的物种测试（即人和大鼠）之间的差异直接反映了重组聚（ADP-核糖）聚合酶-1（PARP-1）的酶参数，即底物亲和力和反应速度。此外，我们已经表征了两个人PARP-1等位基因，并将其活性差异归因于其各自的初始速度而不是底物亲和力。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Poly(ADP-ribosyl)ation is a posttranslational modification, which is involved in many cellular functions, including DNA repair and maintenance of genomic stability, and has also been implicated in cellular and organismal ageing. We have previously reported that maximum poly(ADP-ribosyl)ation capacity in mononuclear blood cells is correlated with mammalian life span. Here we show that the difference between a long-lived and a short-lived species tested (i.e. man and rat) is directly mirrored by the enzymatic parameters of recombinant poly(ADP-ribose) polymerase-1 (PARP-1), i.e. substrate affinity and reaction velocity. In addition, we have characterized two human PARP-1 alleles and assign their activity difference to their respective initial velocity and not substrate affinity. (C) 2010 Elsevier Ireland Ltd. All rights reserved.