Reproductive toxicity of 1-bromoprapane, a newly introduced alternative to ozone layer depleting solvents, in male rats
Reproductive toxicity of 1-bromoprapane, a newly introduced alternative to ozone layer depleting solvents, in male rats
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DOI：
10.1093/toxsci/54.2.416
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发表时间：
2000-04-01
影响因子：
3.8
通讯作者：
Takeuchi, Y
中科院分区：
文献类型：
--
作者：
Ichihara, G;Yu, XZ;Takeuchi, Y
1-溴丙烷是新引入的臭氧消耗溶剂的替代品。RIE的目的是阐明其对雄性大鼠的剂量依赖性生殖毒性。Wistar雄性大鼠36只，随机分为4组，每组9只，分别暴露于200、400、800ppm的1-溴丙烷或单纯新鲜空气中，每天8h，共12周。在暴露12周后，对附睾精子指数进行了评估。取睾丸、附睾腺、精囊、前列腺等脏器称重，进行组织病理学检查。在第IX-XI期生精上皮的基底部，计数VN期的生精细胞、生精小管和残存的精细胞。用放射免疫法测定血浆睾酮水平。睾丸重量没有明显变化，但附睾体、精囊和前列腺重量呈剂量依赖性下降。在200-ppm及以上的最低浓度下，精囊重量显著降低。1-溴丙烷可引起附睾精子数和活动率呈剂量依赖性下降，无尾精子和未成熟头部精子增多。精原细胞、细线期前精母细胞、粗线期精母细胞和圆形精子细胞在第VII期未见明显减少，而在受精后M-XI期，基底膜附近的残存、拉长的精子细胞呈剂量依赖性增加。血浆睾酮水平在800-ppm剂量时显著降低。1-溴丙烷导致受精失败。它的生殖毒性与2-溴丙烷不同，2-溴丙烷专门损害精原细胞。因此，这种溶剂可能对男性有严重的生殖毒性影响，在工作场所应非常谨慎地使用。
1-Bromopropane has been newly introduced as an alternative to ozone-depleting solvents. Rie aimed to clarify its dose-dependent reproductive toxicity in male rats. Thirty-six Wistar male rats were randomly divided into 4 groups of 9. The groups were exposed to 200, 400, or 800 ppm 1-bromopropane or only fresh air, 8 h per day for 12 weeks. Epididymal sperm indices were evaluated after a 12-week exposure. The testes, epididymides, seminal vesicle, prostate, and other organs were weighed and examined histopathologically. Spermatogenic cells, in stage Vn, seminiferous tubules, and retained spermatids, at the basal region of stages IX-XI seminiferous epithelium, were counted. Plasma testosterone levels were measured by radioimmunoassay. The testicular weight did not significantly change, but the weight of epididymides, seminal vesicle, and prostate dose-dependently decreased. The weight of seminal vesicle decreased significantly at the lowest concentration of 200-ppm and over. 1-Bromopropane induced a dose-dependent decrease in the epididymal sperm count and in motility, as well as an increase in tailless sperm and sperm with an immature head shape. The spermatogonia, preleptotene spermatocytes, pachytene spermatocytes, and round spermatids did not decrease significantly at stage VII. Retained, elongated spermatids near the basement membrane at the postspermiation stages M-XI increased dose-dependently. Plasma testosterone levels significantly decreased at the 800-ppm dosage. 1-Bromopropane caused failure of spermiation. Its reproductive toxicity is different from that of 2-bromopropane, which specifically impairs spermatogonia. Thus, this solvent may have serious reproductive toxic effects in men, and should be used very cautiously in the workplace.