THE PROJECTION OF THE SUPRAMAMMILLARY NUCLEUS TO THE HIPPOCAMPAL-FORMATION - AN IMMUNOHISTOCHEMICAL AND ANTEROGRADE TRANSPORT STUDY WITH THE LECTIN PHA-L IN THE RAT
THE PROJECTION OF THE SUPRAMAMMILLARY NUCLEUS TO THE HIPPOCAMPAL-FORMATION - AN IMMUNOHISTOCHEMICAL AND ANTEROGRADE TRANSPORT STUDY WITH THE LECTIN PHA-L IN THE RAT
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DOI：
10.1002/cne.902290204
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发表时间：
1984-01-01
影响因子：
2.5
通讯作者：
KOHLER, C
中科院分区：
文献类型：
--
作者：
HAGLUND, L;SWANSON, LW;KOHLER, C
用免疫组织化学和轴突运输方法研究了成年雄性大鼠乳头体上外侧核向海马结构投射的组织结构和可能的神经递质特异性。逆行示踪剂真蓝的实验表明，在齿状回和阿蒙角的背侧部分或内嗅区注射后，整个核的吻尾侧范围内的神经元都被标记，尽管内嗅注射标记的细胞倾向于占据核的更多腹侧部分。结合化学-逆行转运研究表明，乳头体上核尾侧端有少量（< 5%）胆囊收缩素免疫反应神经元投射到海马结构，整个核中也有一些（5-10%）血管活性肠多肽（VIP）免疫反应神经元投射到海马结构。用凝集素菜豆白细胞凝集素（PHA-L）进行的顺行运输研究表明，来自乳头状核的纤维支配海马结构的所有部分。在齿状回颗粒层和分子层、Ammon角CA 3区分子层、CA 3a区锥体层和定向层内均观察到许多终末终扣的曲张纤维。在CA 1和CA 2区仅发现散在纤维。表观终端领域也观察到在分子层的表面部分，和锥体层的深层部分，下托，在最深层的前下托和parasubiculum，并在所有层的内嗅区。
The organization and possible neurotransmitter specificity of a projection from the lateral supramammillary nucleus to the hippocampal formation was examined with immunohistochemical and axonal transport methods in the adult male rat. Experiments with the retrograde tracer true blue indicate that neurons throughout the rostrocaudal extent of the nucleus are labeled after injections in either dorsal parts of the dentate gyrus and Ammmon''s horn, or the entorhinal area, although cells labeled by the entorhinal injections tended to occupy more ventral parts of the nucleus. Combined immunohistochemical-retrograde transport studies showed that a small number (< 5%) of cholecystokinin-immunoreactive neurons in the caudal tip of the supramammillary nucleus project to the hippocampal formation, as do some (5-10%) vasoactive intestinal polypeptide (VIP)-immunoreactive neurons throughout the nucleus. Anterograde transport studies with the lectin Phaseolus vulgaris leukoagglutinin (PHA-L) indicate that fibers from the supramammilary nucleus innervate all parts of the hippocampal formation. Many varicose fibers with terminal boutons were observed in the granular and molecular layers of the dentate gyrus, throughout the molecular layer of field CA3 of Ammon''s horn, and in the pyramidal layer and stratum oriens of subfield CA3a. Only scattered fibers were found in fields CA1 and CA2. Apparent terminal fields were also observed in superficial parts of the molecular layer, and deep parts of the pyramidal layer, of the subiculum, in the deepest layer of the presubiculum and parasubiculum, and in all layers of the entorhinal area.