POTASSIUM-INDUCED INCREASE IN OXYGEN-CONSUMPTION OF BROWN ADIPOSE-TISSUE FROM RAT
POTASSIUM-INDUCED INCREASE IN OXYGEN-CONSUMPTION OF BROWN ADIPOSE-TISSUE FROM RAT
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DOI：
10.1113/jphysiol.1975.sp011156
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发表时间：
1975-01-01
影响因子：
5.5
通讯作者：
GIRARDIER, L
中科院分区：
文献类型：
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作者：
BARDE, YA;CHINET, A;GIRARDIER, L
1.在棕色脂肪组织中，去甲肾上腺素诱导呼吸增加和细胞去极化。在已知的范围内增加钾浓度对棕色脂肪细胞去角质的影响对O2消耗进行了测试。2.分离的肩胛间棕色脂肪组织从大鼠中孵育室，允许O2消耗量进行测量，在较长的时间内。3.发现45 - 50 mM-KCl可诱导O2消耗量增加4倍以上，这是稳定的、可逆的，并取决于培养基中钙的存在。4.当用利血平或6-羟基多巴胺预处理大鼠时，KCl诱导的O2消耗量增加急剧减少或完全消失。5.（-）-普萘洛尔可显著抑制KC 1的作用，而（+）-普萘洛尔则无此作用。6.适度增加O2消耗诱导低浓度的钾被加强地昔帕明，一种药物，这是已知的阻断肾上腺素能神经末梢的摄取儿茶酚胺。7.手术去神经引起的组织中的儿茶酚胺含量的减少，但没有影响的KC 1反应。8.它的结论是，在棕色脂肪组织，钾刺激O2消耗引起释放去甲肾上腺素从神经末梢。这意味着，手术去神经，因为它是通常在这个组织上进行不去神经的棕色脂肪细胞，但可能只有血管。
1. In brown adipose tissue, noradrenaline induces an increase in respiration and a depolarization of the cells. The effect of an increase in potassium concentration in a range known to depolarize the brown adipocytes was tested on the O2 consumption. 2. Isolated interscapular brown adipose tissue from the rat was incubated in chambers that allowed O2 consumption to be measured over prolonged periods. 3. 45‐50 mM‐KC1 were found to induce a more that fourfold increase in O2 consumption, which was stable, reversible and dependent upon the presence of calcium in the meduim. 4. When rats were pre‐treated with reserpine or 6‐hydroxydopamine the KC1‐induced increase in O2 consumption was sharply reduced or entirely adsent. 5. The effect of KC1 was greatly inhibited by (‐)‐propranolol, but not by (+)‐propranolol. 6. Moderate increases in O2 consumption induced by low concentrations of potassium were potentiated by desipramine, a drug which is known to block the uptake of catecholamines by adrenergic nerve endings. 7. Surgical denervation caused a decrease in the catecholamine content of the tissue, but had no effect on the KC1 response. 8. It is concluded that in brown adipose tissue, potassium stimulates O2 consumption by causing a release of noradrenaline from nerve endings. This implies that surgical denervation as it is commonly performed on this tissue does not denervate the brown adipocytes but probably only the blood vessels.