The effect of metanephros transplantation on blood pressure in anephric rats with induced acute hypotension.
The effect of metanephros transplantation on blood pressure in anephric rats with induced acute hypotension.
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DOI：
10.1093/ndt/gfs006
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发表时间：
2012-09
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通讯作者：
S. Yokote;T. Yokoo;Kei Matsumoto;Y. Utsunomiya;T. Kawamura;T. Hosoya
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文献类型：
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作者：
S. Yokote;T. Yokoo;Kei Matsumoto;Y. Utsunomiya;T. Kawamura;T. Hosoya
背景技术肾脏是维持血压的重要器官。我们之前曾报道过移植的后肾可以再现一些肾功能。本研究的目的是确定移植后肾的代谢功能，特别是维持血压。方法 雄性 Wistar 大鼠单侧肾切除后进行后肾移植（移植组，n = 28）。为了进行比较，我们对 32 只大鼠进行了单侧肾切除术而不进行移植（非移植组，n = 18；血肾切除术对照组，n = 14）。初次手术后两周取出剩余的肾脏，而对照大鼠则进行假手术。静脉滴注盐酸地尔硫卓或快速抽血诱发低血压。在多个时间点对平均动脉压（MAP）进行有创监测并分析血浆肾素活性（PRA）。通过实时聚合酶链反应和免疫组织化学评估后肾的肾素表达。结果移植组后肾表达肾素信使RNA。与未移植组相比，后肾移植显着提高了 PRA 并维持了 MAP。在 PRA 或 MAP 方面，移植组和对照组之间没有发现显着差异。移植组中PRA与后肾重量以及MAP呈正相关。结论 本研究表明，后肾移植可产生 PRA，并有助于提高急性低血压大鼠模型中的 MAP。
BACKGROUND The kidney is an important organ for maintaining blood pressure. We have previously reported that transplanted metanephroi can reproduce some kidney functions. The aim of the present study was to determine the metabolic function of transplanted metanephroi with particular reference to maintaining blood pressure. METHODS Male Wistar rats were transplanted with metanephroi (transplanted group, n = 28), following unilateral nephrectomy. For comparison, we performed unilateral nephrectomy without transplantation in 32 rats (non-transplanted group, n = 18; haeminephrectomy control group, n = 14). The remaining kidney was removed 2 weeks after the initial operation, while control rats had a sham operation. Hypotension was induced by intravenous infusion of diltiazem hydrochloride or rapid withdrawal of blood. Mean arterial blood pressure (MAP) was invasively monitored and plasma renin activity (PRA) was analysed at multiple time points. Renin expression by metanephroi was evaluated by real-time polymerase chain reaction and immunohistochemistry. RESULTS Metanephroi in the transplanted group expressed renin messenger RNA. Metanephros transplantation significantly raised PRA and maintained MAP compared with the non-transplanted group. No significant differences between the transplanted and control groups were found with respect to PRA or MAP. PRA was positively correlated with metanephroi weight as well as MAP in the transplanted group. CONCLUSION The present study shows that transplantation of metanephroi produces PRA and contributes to raising MAP in a rat model of acute hypotension.