THE ROLE OF THE VIMENTIN INTERMEDIATE FILAMENTS IN RAT 3Y1-CELLS ELUCIDATED BY IMMUNOELECTRON MICROSCOPY AND COMPUTER-GRAPHIC RECONSTRUCTION
THE ROLE OF THE VIMENTIN INTERMEDIATE FILAMENTS IN RAT 3Y1-CELLS ELUCIDATED BY IMMUNOELECTRON MICROSCOPY AND COMPUTER-GRAPHIC RECONSTRUCTION
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DOI：
10.1016/0248-4900(90)90299-i
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发表时间：
1990-01-01
影响因子：
2.7
通讯作者：
KURIMURA, T
中科院分区：
文献类型：
--
作者：
KATSUMOTO, T;MITSUSHIMA, A;KURIMURA, T
为探讨波形蛋白中间丝（IF）在成纤维细胞系3Y1中的生物学作用，研究了IF在3Y1细胞中的三维空间排列。虽然肌动蛋白丝只在细胞的表面部分观察到，但波形蛋白IF被发现大量存在于偶尔发现微管的细胞内部。通过免疫电镜和计算机图像重建的连续薄切片，它被观察到更详细的波形蛋白IF位于非常接近的细胞核，内质网和线粒体。观察到波形蛋白IF在这些细胞器的侧面或末端附着。因此，我们可以合理地假设，波形蛋白IF是细胞内的主要细胞骨架结构，它们在支持细胞器的位置方面发挥着重要作用。这是第一个报告，可视化波形蛋白IF和细胞器之间的三维关系。
The three-dimensional arrangement of vimentin intermediate filaments (IF) was studied in 3Y1, rat fibroblast cell line, to elucidate its biological role in thee cell. While actin filaments were observed exclusively in the superficial part of the cell, vimentin IF were found to be abundantly present in the inside of the cell where microtubules were occasionally discovered. By whole-mount immunoelectron microscopy and computer-graphic reconstruction of serial thin sections, it was observed in more detail that vimentin IF are located very close to the nucleus, endoplasmic reticulum, and mitochondria. Vimentin IF were observed to be attached to these organelles laterally or terminally. Thus, we can reasonably assume that vimentin IF are major cytoskeletal structures deep inside the cell and that they play an important role in supporting the location of the organelles. This is the first report which has visualized the three-dimensional relationship between vimentin IF and the organelles of the cell.