Periplasmic dehydroshikimate dehydratase combined with quinate oxidation in Gluconobacter oxydans for protocatechuate production
Periplasmic dehydroshikimate dehydratase combined with quinate oxidation in Gluconobacter oxydans for protocatechuate production
复制标题
氧化葡糖杆菌中周质脱氢莽草酸脱水酶与奎宁酸氧化相结合用于原儿茶酸生产
DOI：
10.1093/bbb/zbac090
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Yakushi T.
中科院分区：
文献类型：
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作者：
Nagaki K;Kataoka N;Theeragool G;Matsutani M;Ano Y;Matsushita K;Yakushi T.
原儿茶酸酯（3,4-二羟基苯甲酸酯）具有抗氧化性能，是生产粘康酸的原料，粘康酸是合成尼龙和聚对苯二甲酸乙二醇酯等聚合物的关键化合物。氧化葡糖杆菌NBRC3244具有氧化奎宁酸生成3-脱氢奎宁酸的周质系统。此前，通过在G.G.的周质中异源表达固氮葡萄糖醋杆菌脱氢奎宁酸脱水酶，构建了周质3-脱氢莽草酸生产系统。 oxydansstrain NBRC3244。 3-脱氢莽草酸通过脱水转化为原儿茶酸。在这项研究中，我们构建了aG。表达贝氏不动杆菌quiC基因的氧化菌株，该基因编码脱氢莽草酸脱水酶，其亚细胞定位可能是周质。我们尝试通过共培养两个重组G来生产原儿茶酸。 oxydansstrains——一种表达周质靶向的脱氢奎酸脱水酶，另一种表达A.月桂酰氢莽草酸脱水酶。共培养系统以近乎定量的方式从奎宁酸中产生原儿茶酸。
Protocatechuate (3,4-dihydroxybenzoate) has antioxidant properties and is a raw material for the production of muconic acid, which is a key compound in the synthesis of polymers such as nylon and polyethylene terephthalate.Gluconobacter oxydansstrain NBRC3244 has a periplasmic system for oxidation of quinate to produce 3-dehydroquinate. Previously, a periplasmic 3-dehydroshikimate production system was constructed by heterologously expressingGluconacetobacter diazotrophicusdehydroquinate dehydratase in the periplasm ofG. oxydansstrain NBRC3244. 3-Dehydroshikimate is converted to protocatechuate by dehydration. In this study, we constructed aG. oxydansstrain that expresses theAcinetobacter baylyi quiCgene, which encodes a dehydroshikimate dehydratase of which the subcellular localization is likely the periplasm. We attempted to produce protocatechuate by co-cultivation of two recombinantG. oxydansstrains—one expressing the periplasmically targeted dehydroquinate dehydratase and the other expressingA. baylyidehydroshikimate dehydratase. The co-cultivation system produced protocatechuate from quinate in a nearly quantitative manner.