Nitrergic and cholinergic innervation of the rat lower urinary tract after pelvic ganglionectomy
Nitrergic and cholinergic innervation of the rat lower urinary tract after pelvic ganglionectomy
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DOI：
10.1152/ajpregu.1998.274.2.r389
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发表时间：
1998-02-01
影响因子：
2.8
通讯作者：
Andersson, KE
中科院分区：
文献类型：
--
作者：
Persson, K;Alm, P;Andersson, KE
采用抗NO合成酶（NOS）和胆碱乙酰转移酶（ChAT）血清双免疫组化方法，探讨了大鼠盆腔神经节（MPG）内一氧化氮（NO）和乙酰胆碱共存的可能性。在双侧MPG冷冻血管切除术后，记录离体膀胱和尿道的平滑肌反应，重点关注去神经超敏感的可能发展。在MPG中，NOS免疫反应性（ir）在大量细胞体中可见，但不像ChAT-ir细胞体那么丰富。双免疫标记显示所有NOS-ir细胞体也显示出ChAT-ir。在切除神经节的膀胱中，电场刺激（EFS）引起的收缩反应明显减少。与对照膀胱相比，切除神经节的大鼠的逼尿肌条对乙醇更敏感，药物浓度的负对数较低，可引起50%的松弛（6.5 +/- 0.04比5.9 +/- 0.07）。在尿道中，神经节切除术几乎消除了no介导的对EFS的松弛反应，而对盐酸3- morpholinosydnon亚胺（SIN-1）的敏感性没有差异。在对照组和切除神经节的大鼠尿道制剂中，SIN-1使鸟苷3′，5′-环单磷酸的组织水平同样增加。提示NOS-ir神经介导大鼠尿道平滑肌张力抑制来源于MPG，并含有ChAT。神经节切除术后尿道未见去神经对氮刺激的超敏反应。
The possible coexistence of nitric oxide (NO) and acetylcholine in the rat major pelvic ganglion (MPG) was examined by double immunohistochemistry using antisera raised against NO synthase (NOS) and choline acetyltransferase (ChAT). The smooth muscle responses of the isolated bladder and urethra were recorded after bilateral cryoganglionectomy of the MPG, focusing on the possible development of denervation supersensitivity. In the MPG, NOS immunoreactivity (ir) was seen in a large number of cell bodies, but it was not as abundant as ChAT-ir cell bodies. Double immunolabeling showed that all NOS-ir cell bodies also displayed ChAT-ir. In ganglionectomized bladders, the electrical field stimulation (EFS)-evoked contractile response was markedly reduced. When compared with control bladders, detrusor strips from ganglionectomized rats were more sensitive to carbachol as revealed by a lower negative logarithm of the drug concentration eliciting 50% relaxation (6.5 +/- 0.04 vs. 5.9 +/- 0.07). In the urethra, the NO-mediated relaxant response to EFS was practically abolished by ganglionectomy, whereas no difference was found in sensitivity to 3-morpholinosydnonimine hydrochloride (SIN-1). SIN-1 produced an equal increase in tissue levels of guanosine 3',5'-cyclic monophosphate in urethral preparations from control and ganglionectomized rats. The results suggest that the NOS-ir nerves that mediate inhibition of rat urethral smooth muscle tone originate from the MPG and contain ChAT. No denervation supersensitivity to nitrergic stimulation was observed in the urethra after ganglionectomy.